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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pestis bv. Antiqua Chromosomal replication initiator protein DnaA (dnaA) CSB-YP433243YAM
CSB-EP433243YAM
CSB-BP433243YAM
CSB-MP433243YAM
CSB-EP433243YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua ATP synthase subunit c (atpE), partial CSB-YP433244YAM1
CSB-EP433244YAM1
CSB-BP433244YAM1
CSB-MP433244YAM1
CSB-EP433244YAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0267 protein YpAngola_A1631 (YpAngola_A1631) CSB-YP433245YAM
CSB-EP433245YAM
CSB-BP433245YAM
CSB-MP433245YAM
CSB-EP433245YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0266 membrane protein YpAngola_A1654 (YpAngola_A1654), partial CSB-YP433246YAM1
CSB-EP433246YAM1
CSB-BP433246YAM1
CSB-MP433246YAM1
CSB-EP433246YAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 50S ribosomal protein L28 (rpmB) CSB-YP433247YAM
CSB-EP433247YAM
CSB-BP433247YAM
CSB-MP433247YAM
CSB-EP433247YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Trans-aconitate 2-methyltransferase (tam) CSB-YP433248YAM
CSB-EP433248YAM
CSB-BP433248YAM
CSB-MP433248YAM
CSB-EP433248YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH) CSB-YP433249YAM
CSB-EP433249YAM
CSB-BP433249YAM
CSB-MP433249YAM
CSB-EP433249YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP433250YAM
CSB-EP433250YAM
CSB-BP433250YAM
CSB-MP433250YAM
CSB-EP433250YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP433251YAM
CSB-EP433251YAM
CSB-BP433251YAM
CSB-MP433251YAM
CSB-EP433251YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Membrane-bound lytic murein transglycosylase C (mltC) CSB-YP433252YAM
CSB-EP433252YAM
CSB-BP433252YAM
CSB-MP433252YAM
CSB-EP433252YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP433253YAM
CSB-EP433253YAM
CSB-BP433253YAM
CSB-MP433253YAM
CSB-EP433253YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP433254YAM
CSB-EP433254YAM
CSB-BP433254YAM
CSB-MP433254YAM
CSB-EP433254YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Flagellar protein FliT (fliT) CSB-YP433255YAM
CSB-EP433255YAM
CSB-BP433255YAM
CSB-MP433255YAM
CSB-EP433255YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP433256YAM
CSB-EP433256YAM
CSB-BP433256YAM
CSB-MP433256YAM
CSB-EP433256YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP433257YAM
CSB-EP433257YAM
CSB-BP433257YAM
CSB-MP433257YAM
CSB-EP433257YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua HTH-type transcriptional regulator iscR (iscR) CSB-YP433258YAM
CSB-EP433258YAM
CSB-BP433258YAM
CSB-MP433258YAM
CSB-EP433258YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua HTH-type transcriptional regulator hdfR (hdfR) CSB-YP433259YAM
CSB-EP433259YAM
CSB-BP433259YAM
CSB-MP433259YAM
CSB-EP433259YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Putative ECA polymerase (wzyE), partial CSB-YP433260YAM
CSB-EP433260YAM
CSB-BP433260YAM
CSB-MP433260YAM
CSB-EP433260YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Electron transport complex protein RnfA (rnfA), partial CSB-YP433261YAM1
CSB-EP433261YAM1
CSB-BP433261YAM1
CSB-MP433261YAM1
CSB-EP433261YAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 50S ribosomal protein L22 (rplV) CSB-YP433262YAM
CSB-EP433262YAM
CSB-BP433262YAM
CSB-MP433262YAM
CSB-EP433262YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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