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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Gluconacetobacter diazotrophicus Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP432723GEN
CSB-EP432723GEN
CSB-BP432723GEN
CSB-MP432723GEN
CSB-EP432723GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Glutamate--tRNA ligase 2 (gltX2) CSB-YP432724GEN
CSB-EP432724GEN
CSB-BP432724GEN
CSB-MP432724GEN
CSB-EP432724GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP432725GEN
CSB-EP432725GEN
CSB-BP432725GEN
CSB-MP432725GEN
CSB-EP432725GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP432726GEN
CSB-EP432726GEN
CSB-BP432726GEN
CSB-MP432726GEN
CSB-EP432726GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Trigger factor (tig) CSB-YP432727GEN
CSB-EP432727GEN
CSB-BP432727GEN
CSB-MP432727GEN
CSB-EP432727GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 30S ribosomal protein S16 (rpsP) CSB-YP432728GEN
CSB-EP432728GEN
CSB-BP432728GEN
CSB-MP432728GEN
CSB-EP432728GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 2-isopropylmalate synthase (leuA), partial CSB-YP432729BPB
CSB-EP432729BPB
CSB-BP432729BPB
CSB-MP432729BPB
CSB-EP432729BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Anthranilate phosphoribosyltransferase (trpD) CSB-YP432730BPB
CSB-EP432730BPB
CSB-BP432730BPB
CSB-MP432730BPB
CSB-EP432730BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Siroheme synthase (cysG) CSB-YP432731BPB
CSB-EP432731BPB
CSB-BP432731BPB
CSB-MP432731BPB
CSB-EP432731BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii UPF0234 protein Bpet3698 (Bpet3698) CSB-YP432732BPB
CSB-EP432732BPB
CSB-BP432732BPB
CSB-MP432732BPB
CSB-EP432732BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii UPF0042 nucleotide-binding protein Bpet0443 (Bpet0443) CSB-YP432733BPB
CSB-EP432733BPB
CSB-BP432733BPB
CSB-MP432733BPB
CSB-EP432733BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Putative Holliday junction resolvase (Bpet0562) CSB-YP432734BPB
CSB-EP432734BPB
CSB-BP432734BPB
CSB-MP432734BPB
CSB-EP432734BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP432735BPB
CSB-EP432735BPB
CSB-BP432735BPB
CSB-MP432735BPB
CSB-EP432735BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Probable 5-dehydro-4-deoxyglucarate dehydratase (Bpet3876) CSB-YP432736BPB
CSB-EP432736BPB
CSB-BP432736BPB
CSB-MP432736BPB
CSB-EP432736BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP432737BPB
CSB-EP432737BPB
CSB-BP432737BPB
CSB-MP432737BPB
CSB-EP432737BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP432738BPB
CSB-EP432738BPB
CSB-BP432738BPB
CSB-MP432738BPB
CSB-EP432738BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Homogentisate 1,2-dioxygenase (hmgA) CSB-YP432739BPB
CSB-EP432739BPB
CSB-BP432739BPB
CSB-MP432739BPB
CSB-EP432739BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii UPF0176 protein Bpet4097 (Bpet4097) CSB-YP432740BPB
CSB-EP432740BPB
CSB-BP432740BPB
CSB-MP432740BPB
CSB-EP432740BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Putative arginyl-tRNA--protein transferase (ate) CSB-YP432741BPB
CSB-EP432741BPB
CSB-BP432741BPB
CSB-MP432741BPB
CSB-EP432741BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Protein-export protein secB (secB) CSB-YP432742BPB
CSB-EP432742BPB
CSB-BP432742BPB
CSB-MP432742BPB
CSB-EP432742BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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