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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella paratyphi B Putative Holliday junction resolvase (yqgF) CSB-YP433123STF
CSB-EP433123STF
CSB-BP433123STF
CSB-MP433123STF
CSB-EP433123STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B DNA mismatch repair protein mutL (mutL), partial CSB-YP433124STF
CSB-EP433124STF
CSB-BP433124STF
CSB-MP433124STF
CSB-EP433124STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Probable L-ascorbate-6-phosphate lactonase ulaG (ulaG) CSB-YP433125STF
CSB-EP433125STF
CSB-BP433125STF
CSB-MP433125STF
CSB-EP433125STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B L-ribulose-5-phosphate 3-epimerase UlaE (ulaE) CSB-YP433126STF
CSB-EP433126STF
CSB-BP433126STF
CSB-MP433126STF
CSB-EP433126STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP433127STF
CSB-EP433127STF
CSB-BP433127STF
CSB-MP433127STF
CSB-EP433127STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH) CSB-YP433128STF
CSB-EP433128STF
CSB-BP433128STF
CSB-MP433128STF
CSB-EP433128STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD) CSB-YP433129STF
CSB-EP433129STF
CSB-BP433129STF
CSB-MP433129STF
CSB-EP433129STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Lipopolysaccharide core heptose (II)-phosphate phosphatase CSB-YP433130STF
CSB-EP433130STF
CSB-BP433130STF
CSB-MP433130STF
CSB-EP433130STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Beta-hexosaminidase (nagZ) CSB-YP433131STF
CSB-EP433131STF
CSB-BP433131STF
CSB-MP433131STF
CSB-EP433131STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Flagellar P-ring protein (flgI) CSB-YP433132STF
CSB-EP433132STF
CSB-BP433132STF
CSB-MP433132STF
CSB-EP433132STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Bifunctional protein HldE (hldE) CSB-YP433133STF
CSB-EP433133STF
CSB-BP433133STF
CSB-MP433133STF
CSB-EP433133STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Putrescine aminotransferase (patA) CSB-YP433134STF
CSB-EP433134STF
CSB-BP433134STF
CSB-MP433134STF
CSB-EP433134STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP433135STF
CSB-EP433135STF
CSB-BP433135STF
CSB-MP433135STF
CSB-EP433135STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Curved DNA-binding protein (cbpA) CSB-YP433136STF
CSB-EP433136STF
CSB-BP433136STF
CSB-MP433136STF
CSB-EP433136STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Dihydroorotate dehydrogenase (quinone) CSB-YP433137STF
CSB-EP433137STF
CSB-BP433137STF
CSB-MP433137STF
CSB-EP433137STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B tRNA pseudouridine synthase B (truB) CSB-YP433138STF
CSB-EP433138STF
CSB-BP433138STF
CSB-MP433138STF
CSB-EP433138STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 50S ribosomal protein L21 (rplU) CSB-YP433139STF
CSB-EP433139STF
CSB-BP433139STF
CSB-MP433139STF
CSB-EP433139STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Primosomal protein 1 (dnaT) CSB-YP433140STF
CSB-EP433140STF
CSB-BP433140STF
CSB-MP433140STF
CSB-EP433140STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Non-canonical purine NTP phosphatase (yjjX) CSB-YP433141STF
CSB-EP433141STF
CSB-BP433141STF
CSB-MP433141STF
CSB-EP433141STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Multidrug resistance protein MdtB (mdtB), partial CSB-YP433142STF
CSB-EP433142STF
CSB-BP433142STF
CSB-MP433142STF
CSB-EP433142STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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