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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bordetella petrii Serine--tRNA ligase (serS) CSB-YP432743BPB
CSB-EP432743BPB
CSB-BP432743BPB
CSB-MP432743BPB
CSB-EP432743BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii S-adenosylmethionine synthase (metK) CSB-YP432744BPB
CSB-EP432744BPB
CSB-BP432744BPB
CSB-MP432744BPB
CSB-EP432744BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Protein CyaY (cyaY) CSB-YP432745BPB
CSB-EP432745BPB
CSB-BP432745BPB
CSB-MP432745BPB
CSB-EP432745BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 30S ribosomal protein S5 (rpsE) CSB-YP432746BPB
CSB-EP432746BPB
CSB-BP432746BPB
CSB-MP432746BPB
CSB-EP432746BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP432747BPB
CSB-EP432747BPB
CSB-BP432747BPB
CSB-MP432747BPB
CSB-EP432747BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Tryptophan synthase beta chain (trpB) CSB-YP432748BPB
CSB-EP432748BPB
CSB-BP432748BPB
CSB-MP432748BPB
CSB-EP432748BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Probable cytosol aminopeptidase (pepA) CSB-YP432749BPB
CSB-EP432749BPB
CSB-BP432749BPB
CSB-MP432749BPB
CSB-EP432749BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L16 (rplP) CSB-YP432750BPB
CSB-EP432750BPB
CSB-BP432750BPB
CSB-MP432750BPB
CSB-EP432750BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 30S ribosomal protein S10 (rpsJ) CSB-YP432751BPB
CSB-EP432751BPB
CSB-BP432751BPB
CSB-MP432751BPB
CSB-EP432751BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L11 (rplK) CSB-YP432752BPB
CSB-EP432752BPB
CSB-BP432752BPB
CSB-MP432752BPB
CSB-EP432752BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Alanine--tRNA ligase (alaS), partial CSB-YP432753BPB
CSB-EP432753BPB
CSB-BP432753BPB
CSB-MP432753BPB
CSB-EP432753BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Adenylosuccinate synthetase (purA) CSB-YP432754BPB
CSB-EP432754BPB
CSB-BP432754BPB
CSB-MP432754BPB
CSB-EP432754BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Flagellar hook-basal body complex protein FliE (fliE) CSB-YP432755BPB
CSB-EP432755BPB
CSB-BP432755BPB
CSB-MP432755BPB
CSB-EP432755BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP432756BOS
CSB-EP432756BOS
CSB-BP432756BOS
CSB-MP432756BOS
CSB-EP432756BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Chaperone protein DnaK (dnaK), partial CSB-YP432757BOS
CSB-EP432757BOS
CSB-BP432757BOS
CSB-MP432757BOS
CSB-EP432757BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Cytidylate kinase (cmk) CSB-YP432758BOS
CSB-EP432758BOS
CSB-BP432758BOS
CSB-MP432758BOS
CSB-EP432758BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 3-dehydroquinate dehydratase (aroQ) CSB-YP432759BOS
CSB-EP432759BOS
CSB-BP432759BOS
CSB-MP432759BOS
CSB-EP432759BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Gamma-glutamyl phosphate reductase (proA) CSB-YP432760BOS
CSB-EP432760BOS
CSB-BP432760BOS
CSB-MP432760BOS
CSB-EP432760BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP432761BOS
CSB-EP432761BOS
CSB-BP432761BOS
CSB-MP432761BOS
CSB-EP432761BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP432762BOS
CSB-EP432762BOS
CSB-BP432762BOS
CSB-MP432762BOS
CSB-EP432762BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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