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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella paratyphi B Protein slyX (slyX) CSB-YP433063STF
CSB-EP433063STF
CSB-BP433063STF
CSB-MP433063STF
CSB-EP433063STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Protein tsgA (tsgA), partial CSB-YP433064STF
CSB-EP433064STF
CSB-BP433064STF
CSB-MP433064STF
CSB-EP433064STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP433065STF
CSB-EP433065STF
CSB-BP433065STF
CSB-MP433065STF
CSB-EP433065STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Biotin synthase (bioB) CSB-YP433066STF
CSB-EP433066STF
CSB-BP433066STF
CSB-MP433066STF
CSB-EP433066STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP433067STF
CSB-EP433067STF
CSB-BP433067STF
CSB-MP433067STF
CSB-EP433067STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Thiosulfate sulfurtransferase glpE (glpE) CSB-YP433068STF
CSB-EP433068STF
CSB-BP433068STF
CSB-MP433068STF
CSB-EP433068STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B D-cysteine desulfhydrase (dcyD) CSB-YP433069STF
CSB-EP433069STF
CSB-BP433069STF
CSB-MP433069STF
CSB-EP433069STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Arginine--tRNA ligase (argS), partial CSB-YP433070STF
CSB-EP433070STF
CSB-BP433070STF
CSB-MP433070STF
CSB-EP433070STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Leucine--tRNA ligase (leuS), partial CSB-YP433071STF
CSB-EP433071STF
CSB-BP433071STF
CSB-MP433071STF
CSB-EP433071STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP433072STF
CSB-EP433072STF
CSB-BP433072STF
CSB-MP433072STF
CSB-EP433072STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Protein yebF (yebF) CSB-YP433073STF
CSB-EP433073STF
CSB-BP433073STF
CSB-MP433073STF
CSB-EP433073STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP433074STF
CSB-EP433074STF
CSB-BP433074STF
CSB-MP433074STF
CSB-EP433074STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP433075STF
CSB-EP433075STF
CSB-BP433075STF
CSB-MP433075STF
CSB-EP433075STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Nucleoid occlusion factor SlmA (slmA) CSB-YP433076STF
CSB-EP433076STF
CSB-BP433076STF
CSB-MP433076STF
CSB-EP433076STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0745 protein YcgL (ycgL) CSB-YP433077STF
CSB-EP433077STF
CSB-BP433077STF
CSB-MP433077STF
CSB-EP433077STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Periplasmic trehalase (treA), partial CSB-YP433078STF
CSB-EP433078STF
CSB-BP433078STF
CSB-MP433078STF
CSB-EP433078STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP433079STF
CSB-EP433079STF
CSB-BP433079STF
CSB-MP433079STF
CSB-EP433079STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 50S ribosomal protein L36 (rpmJ) CSB-YP433080STF
CSB-EP433080STF
CSB-BP433080STF
CSB-MP433080STF
CSB-EP433080STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Chaperone protein TorD (torD) CSB-YP433081STF
CSB-EP433081STF
CSB-BP433081STF
CSB-MP433081STF
CSB-EP433081STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Probable intracellular septation protein A (yciB) CSB-YP433082STF
CSB-EP433082STF
CSB-BP433082STF
CSB-MP433082STF
CSB-EP433082STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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