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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Bartonella tribocorum 50S ribosomal protein L14 (rplN) CSB-YP433663BOS
CSB-EP433663BOS
CSB-BP433663BOS
CSB-MP433663BOS
CSB-EP433663BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 50S ribosomal protein L3 (rplC) CSB-YP433664BOS
CSB-EP433664BOS
CSB-BP433664BOS
CSB-MP433664BOS
CSB-EP433664BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum DNA ligase (ligA), partial CSB-YP433665BOS
CSB-EP433665BOS
CSB-BP433665BOS
CSB-MP433665BOS
CSB-EP433665BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Probable chorismate--pyruvate lyase (ubiC) CSB-YP433666BOS
CSB-EP433666BOS
CSB-BP433666BOS
CSB-MP433666BOS
CSB-EP433666BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Dihydrodipicolinate reductase (dapB) CSB-YP433667BOS
CSB-EP433667BOS
CSB-BP433667BOS
CSB-MP433667BOS
CSB-EP433667BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Phosphoglucosamine mutase (glmM) CSB-YP433668BOS
CSB-EP433668BOS
CSB-BP433668BOS
CSB-MP433668BOS
CSB-EP433668BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Phosphoglycerate kinase (pgk) CSB-YP433669BOS
CSB-EP433669BOS
CSB-BP433669BOS
CSB-MP433669BOS
CSB-EP433669BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP433670BOS
CSB-EP433670BOS
CSB-BP433670BOS
CSB-MP433670BOS
CSB-EP433670BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Latino virus RING finger protein Z (Z) CSB-YP433671LNT
CSB-EP433671LNT
CSB-BP433671LNT
CSB-MP433671LNT
CSB-EP433671LNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Serine protease HTRA1B (htra1b) CSB-YP433672DIL
CSB-EP433672DIL
CSB-BP433672DIL
CSB-MP433672DIL
CSB-EP433672DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial (coq4), partial CSB-YP433673XBE
CSB-EP433673XBE
CSB-BP433673XBE
CSB-MP433673XBE
CSB-EP433673XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phenol-soluble modulin alpha 3 peptide (psmA3) CSB-YP433674SUL
CSB-EP433674SUL
CSB-BP433674SUL
CSB-MP433674SUL
CSB-EP433674SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Cysteine--tRNA ligase (cysS) CSB-YP433675DXO
CSB-EP433675DXO
CSB-BP433675DXO
CSB-MP433675DXO
CSB-EP433675DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP433676DXO
CSB-EP433676DXO
CSB-BP433676DXO
CSB-MP433676DXO
CSB-EP433676DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii N utilization substance protein B homolog (nusB) CSB-YP433677DXO
CSB-EP433677DXO
CSB-BP433677DXO
CSB-MP433677DXO
CSB-EP433677DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP433678DXO
CSB-EP433678DXO
CSB-BP433678DXO
CSB-MP433678DXO
CSB-EP433678DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP433679DXO
CSB-EP433679DXO
CSB-BP433679DXO
CSB-MP433679DXO
CSB-EP433679DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Lipid-A-disaccharide synthase (lpxB) CSB-YP433680DXO
CSB-EP433680DXO
CSB-BP433680DXO
CSB-MP433680DXO
CSB-EP433680DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Adenylosuccinate synthetase (purA) CSB-YP433681DXO
CSB-EP433681DXO
CSB-BP433681DXO
CSB-MP433681DXO
CSB-EP433681DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP433682DXO
CSB-EP433682DXO
CSB-BP433682DXO
CSB-MP433682DXO
CSB-EP433682DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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