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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella baltica Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP435464STN
CSB-EP435464STN
CSB-BP435464STN
CSB-MP435464STN
CSB-EP435464STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glycosyl hydrolase family 109 protein (Sbal195_2933) CSB-YP435465STN
CSB-EP435465STN
CSB-BP435465STN
CSB-MP435465STN
CSB-EP435465STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP435466STN
CSB-EP435466STN
CSB-BP435466STN
CSB-MP435466STN
CSB-EP435466STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP435467STN
CSB-EP435467STN
CSB-BP435467STN
CSB-MP435467STN
CSB-EP435467STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA (uracil (54)-C (5))-methyltransferase CSB-YP435468STN
CSB-EP435468STN
CSB-BP435468STN
CSB-MP435468STN
CSB-EP435468STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L1 (rplA) CSB-YP435469STN
CSB-EP435469STN
CSB-BP435469STN
CSB-MP435469STN
CSB-EP435469STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S7 (rpsG) CSB-YP435470STN
CSB-EP435470STN
CSB-BP435470STN
CSB-MP435470STN
CSB-EP435470STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L2 (rplB) CSB-YP435471STN
CSB-EP435471STN
CSB-BP435471STN
CSB-MP435471STN
CSB-EP435471STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S17 (rpsQ) CSB-YP435472STN
CSB-EP435472STN
CSB-BP435472STN
CSB-MP435472STN
CSB-EP435472STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S8 (rpsH) CSB-YP435473STN
CSB-EP435473STN
CSB-BP435473STN
CSB-MP435473STN
CSB-EP435473STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S11 (rpsK) CSB-YP435474STN
CSB-EP435474STN
CSB-BP435474STN
CSB-MP435474STN
CSB-EP435474STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP435475STN
CSB-EP435475STN
CSB-BP435475STN
CSB-MP435475STN
CSB-EP435475STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica GTPase Der (der) CSB-YP435476STN
CSB-EP435476STN
CSB-BP435476STN
CSB-MP435476STN
CSB-EP435476STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP435477STN
CSB-EP435477STN
CSB-BP435477STN
CSB-MP435477STN
CSB-EP435477STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0761 membrane protein Sbal195_0319 (Sbal195_0319), partial CSB-YP435478STN1
CSB-EP435478STN1
CSB-BP435478STN1
CSB-MP435478STN1
CSB-EP435478STN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Phosphate acyltransferase (plsX) CSB-YP435479STN
CSB-EP435479STN
CSB-BP435479STN
CSB-MP435479STN
CSB-EP435479STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0597 protein Sbal195_3223 (Sbal195_3223) CSB-YP435480STN
CSB-EP435480STN
CSB-BP435480STN
CSB-MP435480STN
CSB-EP435480STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP435481STN
CSB-EP435481STN
CSB-BP435481STN
CSB-MP435481STN
CSB-EP435481STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP435482STN
CSB-EP435482STN
CSB-BP435482STN
CSB-MP435482STN
CSB-EP435482STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Biosynthetic arginine decarboxylase (speA), partial CSB-YP435483STN
CSB-EP435483STN
CSB-BP435483STN
CSB-MP435483STN
CSB-EP435483STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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