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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia phymatum 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP450596BXT
CSB-EP450596BXT
CSB-BP450596BXT
CSB-MP450596BXT
CSB-EP450596BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase (mhpC) CSB-YP450597BXT
CSB-EP450597BXT
CSB-BP450597BXT
CSB-MP450597BXT
CSB-EP450597BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Flagellar transcriptional regulator FlhC (flhC) CSB-YP450598BXT
CSB-EP450598BXT
CSB-BP450598BXT
CSB-MP450598BXT
CSB-EP450598BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Ribulose bisphosphate carboxylase large chain (cbbL), partial CSB-YP450599BXT
CSB-EP450599BXT
CSB-BP450599BXT
CSB-MP450599BXT
CSB-EP450599BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Nucleoid occlusion factor SlmA (slmA) CSB-YP450600YAW
CSB-EP450600YAW
CSB-BP450600YAW
CSB-MP450600YAW
CSB-EP450600YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP450601YAW
CSB-EP450601YAW
CSB-BP450601YAW
CSB-MP450601YAW
CSB-EP450601YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Cell division inhibitor SulA (sulA) CSB-YP450602YAW
CSB-EP450602YAW
CSB-BP450602YAW
CSB-MP450602YAW
CSB-EP450602YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Outer membrane protein assembly factor yaeT (yaeT), partial CSB-YP450603YAW
CSB-EP450603YAW
CSB-BP450603YAW
CSB-MP450603YAW
CSB-EP450603YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP450604YAW
CSB-EP450604YAW
CSB-BP450604YAW
CSB-MP450604YAW
CSB-EP450604YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB DNA mismatch repair protein mutH (mutH) CSB-YP450605YAW
CSB-EP450605YAW
CSB-BP450605YAW
CSB-MP450605YAW
CSB-EP450605YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Bifunctional protein aas (aas), partial CSB-YP450606YAW1
CSB-EP450606YAW1
CSB-BP450606YAW1
CSB-MP450606YAW1
CSB-EP450606YAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L31 (rpmE) CSB-YP450607YAW
CSB-EP450607YAW
CSB-BP450607YAW
CSB-MP450607YAW
CSB-EP450607YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB tRNA (uracil (54)-C (5))-methyltransferase CSB-YP450608YAW
CSB-EP450608YAW
CSB-BP450608YAW
CSB-MP450608YAW
CSB-EP450608YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP450609YAW
CSB-EP450609YAW
CSB-BP450609YAW
CSB-MP450609YAW
CSB-EP450609YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB ATP-dependent RNA helicase rhlB (rhlB) CSB-YP450610YAW
CSB-EP450610YAW
CSB-BP450610YAW
CSB-MP450610YAW
CSB-EP450610YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Diaminopimelate epimerase (dapF) CSB-YP450611YAW
CSB-EP450611YAW
CSB-BP450611YAW
CSB-MP450611YAW
CSB-EP450611YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP450612YAW
CSB-EP450612YAW
CSB-BP450612YAW
CSB-MP450612YAW
CSB-EP450612YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0149 protein YPTS_3318 (YPTS_3318) CSB-YP450613YAW
CSB-EP450613YAW
CSB-BP450613YAW
CSB-MP450613YAW
CSB-EP450613YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Putative Holliday junction resolvase (YPTS_3342) CSB-YP450614YAW
CSB-EP450614YAW
CSB-BP450614YAW
CSB-MP450614YAW
CSB-EP450614YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L11 (rplK) CSB-YP450615YAW
CSB-EP450615YAW
CSB-BP450615YAW
CSB-MP450615YAW
CSB-EP450615YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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