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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Francisella tularensis subsp. mediasiatica Glycerol kinase (glpK), partial CSB-YP450796FFF
CSB-EP450796FFF
CSB-BP450796FFF
CSB-MP450796FFF
CSB-EP450796FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica UPF0102 protein FTM_0486 (FTM_0486) CSB-YP450797FFF
CSB-EP450797FFF
CSB-BP450797FFF
CSB-MP450797FFF
CSB-EP450797FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP450798FFF
CSB-EP450798FFF
CSB-BP450798FFF
CSB-MP450798FFF
CSB-EP450798FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica 50S ribosomal protein L1 (rplA) CSB-YP450799FFF
CSB-EP450799FFF
CSB-BP450799FFF
CSB-MP450799FFF
CSB-EP450799FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica tRNA (guanine-N (1)-)-methyltransferase CSB-YP450800FFF
CSB-EP450800FFF
CSB-BP450800FFF
CSB-MP450800FFF
CSB-EP450800FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP450801FFF
CSB-EP450801FFF
CSB-BP450801FFF
CSB-MP450801FFF
CSB-EP450801FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP450802FFF
CSB-EP450802FFF
CSB-BP450802FFF
CSB-MP450802FFF
CSB-EP450802FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Ribonuclease H (rnhA) CSB-YP450803FFF
CSB-EP450803FFF
CSB-BP450803FFF
CSB-MP450803FFF
CSB-EP450803FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP450804FFF
CSB-EP450804FFF
CSB-BP450804FFF
CSB-MP450804FFF
CSB-EP450804FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica DNA replication and repair protein recF (recF) CSB-YP450805FFF
CSB-EP450805FFF
CSB-BP450805FFF
CSB-MP450805FFF
CSB-EP450805FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica 50S ribosomal protein L9 (rplI) CSB-YP450806FFF
CSB-EP450806FFF
CSB-BP450806FFF
CSB-MP450806FFF
CSB-EP450806FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica UPF0145 protein FTM_0995 (FTM_0995) CSB-YP450807FFF
CSB-EP450807FFF
CSB-BP450807FFF
CSB-MP450807FFF
CSB-EP450807FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Recombination protein RecR (recR) CSB-YP450808FFF
CSB-EP450808FFF
CSB-BP450808FFF
CSB-MP450808FFF
CSB-EP450808FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP450809FFF
CSB-EP450809FFF
CSB-BP450809FFF
CSB-MP450809FFF
CSB-EP450809FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae DNA mismatch repair protein mutL (mutL), partial CSB-YP450810XBC
CSB-EP450810XBC
CSB-BP450810XBC
CSB-MP450810XBC
CSB-EP450810XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Error-prone DNA polymerase (dnaE2), partial CSB-YP450811XBC
CSB-EP450811XBC
CSB-BP450811XBC
CSB-MP450811XBC
CSB-EP450811XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP450812XBC
CSB-EP450812XBC
CSB-BP450812XBC
CSB-MP450812XBC
CSB-EP450812XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 50S ribosomal protein L9 (rplI) CSB-YP450813XBC
CSB-EP450813XBC
CSB-BP450813XBC
CSB-MP450813XBC
CSB-EP450813XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP450814XBC
CSB-EP450814XBC
CSB-BP450814XBC
CSB-MP450814XBC
CSB-EP450814XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP450815XBC
CSB-EP450815XBC
CSB-BP450815XBC
CSB-MP450815XBC
CSB-EP450815XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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