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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Erwinia tasmaniensis UPF0176 protein ETA_20590 (ETA_20590) CSB-YP451136ENB
CSB-EP451136ENB
CSB-BP451136ENB
CSB-MP451136ENB
CSB-EP451136ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Flagellar hook-basal body complex protein FliE (fliE) CSB-YP451137ENB
CSB-EP451137ENB
CSB-BP451137ENB
CSB-MP451137ENB
CSB-EP451137ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP451138ENB
CSB-EP451138ENB
CSB-BP451138ENB
CSB-MP451138ENB
CSB-EP451138ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP451139ENB
CSB-EP451139ENB
CSB-BP451139ENB
CSB-MP451139ENB
CSB-EP451139ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP451140ENB
CSB-EP451140ENB
CSB-BP451140ENB
CSB-MP451140ENB
CSB-EP451140ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP451141ENB
CSB-EP451141ENB
CSB-BP451141ENB
CSB-MP451141ENB
CSB-EP451141ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis N-succinylarginine dihydrolase (astB) CSB-YP451142ENB
CSB-EP451142ENB
CSB-BP451142ENB
CSB-MP451142ENB
CSB-EP451142ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Electron transport complex protein RnfA (rnfA), partial CSB-YP451143ENB1
CSB-EP451143ENB1
CSB-BP451143ENB1
CSB-MP451143ENB1
CSB-EP451143ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Ribulokinase (araB), partial CSB-YP451144ENB
CSB-EP451144ENB
CSB-BP451144ENB
CSB-MP451144ENB
CSB-EP451144ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Putative reductase ETA_17430 (ETA_17430) CSB-YP451145ENB
CSB-EP451145ENB
CSB-BP451145ENB
CSB-MP451145ENB
CSB-EP451145ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Chromosome initiation inhibitor (iciA) CSB-YP451146ENB
CSB-EP451146ENB
CSB-BP451146ENB
CSB-MP451146ENB
CSB-EP451146ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis ATP-dependent protease subunit HslV (hslV) CSB-YP451147ENB
CSB-EP451147ENB
CSB-BP451147ENB
CSB-MP451147ENB
CSB-EP451147ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Orotate phosphoribosyltransferase (pyrE) CSB-YP451148ENB
CSB-EP451148ENB
CSB-BP451148ENB
CSB-MP451148ENB
CSB-EP451148ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Protein viaA (viaA) CSB-YP451149ENB
CSB-EP451149ENB
CSB-BP451149ENB
CSB-MP451149ENB
CSB-EP451149ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP451150ENB
CSB-EP451150ENB
CSB-BP451150ENB
CSB-MP451150ENB
CSB-EP451150ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Protein syd (syd) CSB-YP451151ENB
CSB-EP451151ENB
CSB-BP451151ENB
CSB-MP451151ENB
CSB-EP451151ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Cell division protein FtsB (ftsB), partial CSB-YP451152ENB
CSB-EP451152ENB
CSB-BP451152ENB
CSB-MP451152ENB
CSB-EP451152ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP451153ENB
CSB-EP451153ENB
CSB-BP451153ENB
CSB-MP451153ENB
CSB-EP451153ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP451154ENB
CSB-EP451154ENB
CSB-BP451154ENB
CSB-MP451154ENB
CSB-EP451154ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis ATP-dependent RNA helicase rhlB (rhlB) CSB-YP451155ENB
CSB-EP451155ENB
CSB-BP451155ENB
CSB-MP451155ENB
CSB-EP451155ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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