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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Polynucleobacter necessarius UPF0234 protein Pnec_0318 (Pnec_0318) CSB-YP450055EXM
CSB-EP450055EXM
CSB-BP450055EXM
CSB-MP450055EXM
CSB-EP450055EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 30S ribosomal protein S6 (rpsF) CSB-YP450056EXM
CSB-EP450056EXM
CSB-BP450056EXM
CSB-MP450056EXM
CSB-EP450056EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 30S ribosomal protein S2 (rpsB) CSB-YP450057EXM
CSB-EP450057EXM
CSB-BP450057EXM
CSB-MP450057EXM
CSB-EP450057EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Recombination protein RecR (recR) CSB-YP450058EXM
CSB-EP450058EXM
CSB-BP450058EXM
CSB-MP450058EXM
CSB-EP450058EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Bifunctional protein FolD (folD) CSB-YP450059EXM
CSB-EP450059EXM
CSB-BP450059EXM
CSB-MP450059EXM
CSB-EP450059EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP450060EXM
CSB-EP450060EXM
CSB-BP450060EXM
CSB-MP450060EXM
CSB-EP450060EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP450061EXM
CSB-EP450061EXM
CSB-BP450061EXM
CSB-MP450061EXM
CSB-EP450061EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP450062EXM
CSB-EP450062EXM
CSB-BP450062EXM
CSB-MP450062EXM
CSB-EP450062EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Threonine--tRNA ligase (thrS), partial CSB-YP450063EXM
CSB-EP450063EXM
CSB-BP450063EXM
CSB-MP450063EXM
CSB-EP450063EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Dihydroorotate dehydrogenase (quinone) CSB-YP450064EXM
CSB-EP450064EXM
CSB-BP450064EXM
CSB-MP450064EXM
CSB-EP450064EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Pantothenate synthetase (panC) CSB-YP450065EXM
CSB-EP450065EXM
CSB-BP450065EXM
CSB-MP450065EXM
CSB-EP450065EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 30S ribosomal protein S21 (rpsU) CSB-YP450066EXM
CSB-EP450066EXM
CSB-BP450066EXM
CSB-MP450066EXM
CSB-EP450066EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L33 (rpmG) CSB-YP450067EXM
CSB-EP450067EXM
CSB-BP450067EXM
CSB-MP450067EXM
CSB-EP450067EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Chorismate synthase (aroC) CSB-YP450068LOT
CSB-EP450068LOT
CSB-BP450068LOT
CSB-MP450068LOT
CSB-EP450068LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Ferredoxin--NADP reductase (Lcho_2791) CSB-YP450069LOT
CSB-EP450069LOT
CSB-BP450069LOT
CSB-MP450069LOT
CSB-EP450069LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP450070LOT
CSB-EP450070LOT
CSB-BP450070LOT
CSB-MP450070LOT
CSB-EP450070LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP450071LOT
CSB-EP450071LOT
CSB-BP450071LOT
CSB-MP450071LOT
CSB-EP450071LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 30S ribosomal protein S2 (rpsB) CSB-YP450072LOT
CSB-EP450072LOT
CSB-BP450072LOT
CSB-MP450072LOT
CSB-EP450072LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Leucine--tRNA ligase (leuS), partial CSB-YP450073LOT
CSB-EP450073LOT
CSB-BP450073LOT
CSB-MP450073LOT
CSB-EP450073LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP450074LOT
CSB-EP450074LOT
CSB-BP450074LOT
CSB-MP450074LOT
CSB-EP450074LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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