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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype IB NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP450656YAW
CSB-EP450656YAW
CSB-BP450656YAW
CSB-MP450656YAW
CSB-EP450656YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Bifunctional protein GlmU (glmU) CSB-YP450657YAW
CSB-EP450657YAW
CSB-BP450657YAW
CSB-MP450657YAW
CSB-EP450657YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB LPS-assembly lipoprotein lptE (lptE) CSB-YP450658YAW
CSB-EP450658YAW
CSB-BP450658YAW
CSB-MP450658YAW
CSB-EP450658YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Glucosamine-6-phosphate deaminase (nagB) CSB-YP450659YAW
CSB-EP450659YAW
CSB-BP450659YAW
CSB-MP450659YAW
CSB-EP450659YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP450660YAW
CSB-EP450660YAW
CSB-BP450660YAW
CSB-MP450660YAW
CSB-EP450660YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Putative sialic acid transporter (nanT), partial CSB-YP450661YAW
CSB-EP450661YAW
CSB-BP450661YAW
CSB-MP450661YAW
CSB-EP450661YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0370 protein YPTS_2881 (YPTS_2881), partial CSB-YP450662YAW
CSB-EP450662YAW
CSB-BP450662YAW
CSB-MP450662YAW
CSB-EP450662YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Nucleoid-associated protein YPTS_1390 (YPTS_1390) CSB-YP450663YAW
CSB-EP450663YAW
CSB-BP450663YAW
CSB-MP450663YAW
CSB-EP450663YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP450664YAW
CSB-EP450664YAW
CSB-BP450664YAW
CSB-MP450664YAW
CSB-EP450664YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Co-chaperone protein hscB (hscB) CSB-YP450665YAW
CSB-EP450665YAW
CSB-BP450665YAW
CSB-MP450665YAW
CSB-EP450665YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Cytidylate kinase (cmk) CSB-YP450666YAW
CSB-EP450666YAW
CSB-BP450666YAW
CSB-MP450666YAW
CSB-EP450666YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP450667YAW
CSB-EP450667YAW
CSB-BP450667YAW
CSB-MP450667YAW
CSB-EP450667YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Pyrrolidone-carboxylate peptidase (pcp) CSB-YP450668YAW
CSB-EP450668YAW
CSB-BP450668YAW
CSB-MP450668YAW
CSB-EP450668YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Urease accessory protein UreD (ureD) CSB-YP450669YAW
CSB-EP450669YAW
CSB-BP450669YAW
CSB-MP450669YAW
CSB-EP450669YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Ribonuclease H (rnhA) CSB-YP450670YAW
CSB-EP450670YAW
CSB-BP450670YAW
CSB-MP450670YAW
CSB-EP450670YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum 50S ribosomal protein L28 (rpmB) CSB-YP450671EKE
CSB-EP450671EKE
CSB-BP450671EKE
CSB-MP450671EKE
CSB-EP450671EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum DNA mismatch repair protein MutS (mutS), partial CSB-YP450672EKE
CSB-EP450672EKE
CSB-BP450672EKE
CSB-MP450672EKE
CSB-EP450672EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP450673EKE
CSB-EP450673EKE
CSB-BP450673EKE
CSB-MP450673EKE
CSB-EP450673EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP450674EKE
CSB-EP450674EKE
CSB-BP450674EKE
CSB-MP450674EKE
CSB-EP450674EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elusimicrobium minutum Ribosome maturation factor RimP (rimP) CSB-YP450675EKE
CSB-EP450675EKE
CSB-BP450675EKE
CSB-MP450675EKE
CSB-EP450675EKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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