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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia phytofirmans D-alanine--D-alanine ligase (ddl) CSB-YP450856BXU
CSB-EP450856BXU
CSB-BP450856BXU
CSB-MP450856BXU
CSB-EP450856BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 50S ribosomal protein L20 (rplT) CSB-YP450857BXU
CSB-EP450857BXU
CSB-BP450857BXU
CSB-MP450857BXU
CSB-EP450857BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Phosphoglucosamine mutase (glmM) CSB-YP450858BXU
CSB-EP450858BXU
CSB-BP450858BXU
CSB-MP450858BXU
CSB-EP450858BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP450859BXU
CSB-EP450859BXU
CSB-BP450859BXU
CSB-MP450859BXU
CSB-EP450859BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Putative Holliday junction resolvase (Bphyt_0869) CSB-YP450860BXU
CSB-EP450860BXU
CSB-BP450860BXU
CSB-MP450860BXU
CSB-EP450860BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Aspartate 1-decarboxylase (panD) CSB-YP450861BXU
CSB-EP450861BXU
CSB-BP450861BXU
CSB-MP450861BXU
CSB-EP450861BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP450862BXU
CSB-EP450862BXU
CSB-BP450862BXU
CSB-MP450862BXU
CSB-EP450862BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP450863BXU
CSB-EP450863BXU
CSB-BP450863BXU
CSB-MP450863BXU
CSB-EP450863BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP450864BXU1
CSB-EP450864BXU1
CSB-BP450864BXU1
CSB-MP450864BXU1
CSB-EP450864BXU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP450865BXU
CSB-EP450865BXU
CSB-BP450865BXU
CSB-MP450865BXU
CSB-EP450865BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans N-succinylarginine dihydrolase (astB) CSB-YP450866BXU
CSB-EP450866BXU
CSB-BP450866BXU
CSB-MP450866BXU
CSB-EP450866BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Putative arginyl-tRNA--protein transferase (ate) CSB-YP450867BXU
CSB-EP450867BXU
CSB-BP450867BXU
CSB-MP450867BXU
CSB-EP450867BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 30S ribosomal protein S18 (rpsR) CSB-YP450868BXU
CSB-EP450868BXU
CSB-BP450868BXU
CSB-MP450868BXU
CSB-EP450868BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP450869BXU
CSB-EP450869BXU
CSB-BP450869BXU
CSB-MP450869BXU
CSB-EP450869BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Alanine racemase (alr) CSB-YP450870BXU
CSB-EP450870BXU
CSB-BP450870BXU
CSB-MP450870BXU
CSB-EP450870BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 50S ribosomal protein L19 (rplS) CSB-YP450871BXU
CSB-EP450871BXU
CSB-BP450871BXU
CSB-MP450871BXU
CSB-EP450871BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Cytidylate kinase (cmk) CSB-YP450872BXU
CSB-EP450872BXU
CSB-BP450872BXU
CSB-MP450872BXU
CSB-EP450872BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP450873BXU
CSB-EP450873BXU
CSB-BP450873BXU
CSB-MP450873BXU
CSB-EP450873BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UPF0758 protein Bphyt_3148 (Bphyt_3148) CSB-YP450874BXU
CSB-EP450874BXU
CSB-BP450874BXU
CSB-MP450874BXU
CSB-EP450874BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 3-deoxy-manno-octulosonate cytidylyltransferase 1 (kdsB1) CSB-YP450875BXU
CSB-EP450875BXU
CSB-BP450875BXU
CSB-MP450875BXU
CSB-EP450875BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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