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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sulfurihydrogenibium sp. 50S ribosomal protein L2 (rplB) CSB-YP451116FPP
CSB-EP451116FPP
CSB-BP451116FPP
CSB-MP451116FPP
CSB-EP451116FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 30S ribosomal protein S12 (rpsL) CSB-YP451117FPP
CSB-EP451117FPP
CSB-BP451117FPP
CSB-MP451117FPP
CSB-EP451117FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP451118FPP
CSB-EP451118FPP
CSB-BP451118FPP
CSB-MP451118FPP
CSB-EP451118FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 60 kDa chaperonin (groL), partial CSB-YP451119FPP
CSB-EP451119FPP
CSB-BP451119FPP
CSB-MP451119FPP
CSB-EP451119FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Probable transcriptional regulatory protein SYO3AOP1_0685 (SYO3AOP1_0685) CSB-YP451120FPP
CSB-EP451120FPP
CSB-BP451120FPP
CSB-MP451120FPP
CSB-EP451120FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP451121FPP
CSB-EP451121FPP
CSB-BP451121FPP
CSB-MP451121FPP
CSB-EP451121FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP451122FPP
CSB-EP451122FPP
CSB-BP451122FPP
CSB-MP451122FPP
CSB-EP451122FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Enolase (eno) CSB-YP451123FPP
CSB-EP451123FPP
CSB-BP451123FPP
CSB-MP451123FPP
CSB-EP451123FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. SsrA-binding protein (smpB) CSB-YP451124FPP
CSB-EP451124FPP
CSB-BP451124FPP
CSB-MP451124FPP
CSB-EP451124FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) CSB-YP451125ENB
CSB-EP451125ENB
CSB-BP451125ENB
CSB-MP451125ENB
CSB-EP451125ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Probable rRNA maturation factor (ETA_23390) CSB-YP451126ENB
CSB-EP451126ENB
CSB-BP451126ENB
CSB-MP451126ENB
CSB-EP451126ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UvrABC system protein B (uvrB), partial CSB-YP451127ENB
CSB-EP451127ENB
CSB-BP451127ENB
CSB-MP451127ENB
CSB-EP451127ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP451128ENB
CSB-EP451128ENB
CSB-BP451128ENB
CSB-MP451128ENB
CSB-EP451128ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Cytidylate kinase (cmk) CSB-YP451129ENB
CSB-EP451129ENB
CSB-BP451129ENB
CSB-MP451129ENB
CSB-EP451129ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis ATP synthase gamma chain (atpG) CSB-YP451130ENB
CSB-EP451130ENB
CSB-BP451130ENB
CSB-MP451130ENB
CSB-EP451130ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Chromosomal replication initiator protein DnaA (dnaA) CSB-YP451131ENB
CSB-EP451131ENB
CSB-BP451131ENB
CSB-MP451131ENB
CSB-EP451131ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP451132ENB
CSB-EP451132ENB
CSB-BP451132ENB
CSB-MP451132ENB
CSB-EP451132ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP451133ENB
CSB-EP451133ENB
CSB-BP451133ENB
CSB-MP451133ENB
CSB-EP451133ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Secretion monitor (secM) CSB-YP451134ENB
CSB-EP451134ENB
CSB-BP451134ENB
CSB-MP451134ENB
CSB-EP451134ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Modulator protein MzrA (mzrA), partial CSB-YP451135ENB
CSB-EP451135ENB
CSB-BP451135ENB
CSB-MP451135ENB
CSB-EP451135ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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