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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum 50S ribosomal protein L9 (rplI) CSB-YP450916DUG
CSB-EP450916DUG
CSB-BP450916DUG
CSB-MP450916DUG
CSB-EP450916DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Arginine repressor (argR) CSB-YP450917DUG
CSB-EP450917DUG
CSB-BP450917DUG
CSB-MP450917DUG
CSB-EP450917DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP450918DUG
CSB-EP450918DUG
CSB-BP450918DUG
CSB-MP450918DUG
CSB-EP450918DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L20 (rplT) CSB-YP450919DUG
CSB-EP450919DUG
CSB-BP450919DUG
CSB-MP450919DUG
CSB-EP450919DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 tRNA 2-selenouridine synthase (selU) CSB-YP450920SYZ
CSB-EP450920SYZ
CSB-BP450920SYZ
CSB-MP450920SYZ
CSB-EP450920SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase (citX) CSB-YP450921SYZ
CSB-EP450921SYZ
CSB-BP450921SYZ
CSB-MP450921SYZ
CSB-EP450921SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Potassium-transporting ATPase B chain (kdpB), partial CSB-YP450922SYZ1
CSB-EP450922SYZ1
CSB-BP450922SYZ1
CSB-MP450922SYZ1
CSB-EP450922SYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP450923SYZ
CSB-EP450923SYZ
CSB-BP450923SYZ
CSB-MP450923SYZ
CSB-EP450923SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L33 (rpmG) CSB-YP450924SYZ
CSB-EP450924SYZ
CSB-BP450924SYZ
CSB-MP450924SYZ
CSB-EP450924SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 4-alpha-L-fucosyltransferase (wecF) CSB-YP450925SYZ
CSB-EP450925SYZ
CSB-BP450925SYZ
CSB-MP450925SYZ
CSB-EP450925SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Dihydroxy-acid dehydratase (ilvD), partial CSB-YP450926SYZ
CSB-EP450926SYZ
CSB-BP450926SYZ
CSB-MP450926SYZ
CSB-EP450926SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP450927SYZ
CSB-EP450927SYZ
CSB-BP450927SYZ
CSB-MP450927SYZ
CSB-EP450927SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Protein TolB (tolB) CSB-YP450928SYZ
CSB-EP450928SYZ
CSB-BP450928SYZ
CSB-MP450928SYZ
CSB-EP450928SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Integration host factor subunit beta (ihfB) CSB-YP450929SYZ
CSB-EP450929SYZ
CSB-BP450929SYZ
CSB-MP450929SYZ
CSB-EP450929SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Hydroxylamine reductase (hcp), partial CSB-YP450930SYZ
CSB-EP450930SYZ
CSB-BP450930SYZ
CSB-MP450930SYZ
CSB-EP450930SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 tRNA modification GTPase MnmE (mnmE) CSB-YP450931SYZ
CSB-EP450931SYZ
CSB-BP450931SYZ
CSB-MP450931SYZ
CSB-EP450931SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Putative transport protein YidE (yidE), partial CSB-YP450932SYZ
CSB-EP450932SYZ
CSB-BP450932SYZ
CSB-MP450932SYZ
CSB-EP450932SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP450933SYZ
CSB-EP450933SYZ
CSB-BP450933SYZ
CSB-MP450933SYZ
CSB-EP450933SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Periplasmic nitrate reductase (napA), partial CSB-YP450934SYZ
CSB-EP450934SYZ
CSB-BP450934SYZ
CSB-MP450934SYZ
CSB-EP450934SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP450935SYZ
CSB-EP450935SYZ
CSB-BP450935SYZ
CSB-MP450935SYZ
CSB-EP450935SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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