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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Kocuria rhizophila 50S ribosomal protein L35 (rpmI) CSB-YP450375KBO
CSB-EP450375KBO
CSB-BP450375KBO
CSB-MP450375KBO
CSB-EP450375KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kocuria rhizophila UPF0182 protein KRH_08700 (KRH_08700), partial CSB-YP450376KBO
CSB-EP450376KBO
CSB-BP450376KBO
CSB-MP450376KBO
CSB-EP450376KBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L14 (rplN) CSB-YP450377MVS
CSB-EP450377MVS
CSB-BP450377MVS
CSB-MP450377MVS
CSB-EP450377MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L30 (rpmD) CSB-YP450378MVS
CSB-EP450378MVS
CSB-BP450378MVS
CSB-MP450378MVS
CSB-EP450378MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_1059 (MMAR_1059) CSB-YP450379MVS
CSB-EP450379MVS
CSB-BP450379MVS
CSB-MP450379MVS
CSB-EP450379MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L17 (rplQ) CSB-YP450380MVS
CSB-EP450380MVS
CSB-BP450380MVS
CSB-MP450380MVS
CSB-EP450380MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum CinA-like protein (MMAR_2796) CSB-YP450381MVS
CSB-EP450381MVS
CSB-BP450381MVS
CSB-MP450381MVS
CSB-EP450381MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Trehalose-phosphate phosphatase (otsB) CSB-YP450382MVS
CSB-EP450382MVS
CSB-BP450382MVS
CSB-MP450382MVS
CSB-EP450382MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum UPF0235 protein MMAR_2910 (MMAR_2910) CSB-YP450383MVS
CSB-EP450383MVS
CSB-BP450383MVS
CSB-MP450383MVS
CSB-EP450383MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_4570 (MMAR_4570) CSB-YP450384MVS
CSB-EP450384MVS
CSB-BP450384MVS
CSB-MP450384MVS
CSB-EP450384MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP450385MVS
CSB-EP450385MVS
CSB-BP450385MVS
CSB-MP450385MVS
CSB-EP450385MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP450386MVS
CSB-EP450386MVS
CSB-BP450386MVS
CSB-MP450386MVS
CSB-EP450386MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP450387MVS
CSB-EP450387MVS
CSB-BP450387MVS
CSB-MP450387MVS
CSB-EP450387MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Cobalamin synthase (cobS), partial CSB-YP450388MVS
CSB-EP450388MVS
CSB-BP450388MVS
CSB-MP450388MVS
CSB-EP450388MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Acetaldehyde dehydrogenase 2 (MMAR_5022) CSB-YP450389MVS
CSB-EP450389MVS
CSB-BP450389MVS
CSB-MP450389MVS
CSB-EP450389MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP450390MVS
CSB-EP450390MVS
CSB-BP450390MVS
CSB-MP450390MVS
CSB-EP450390MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum p-hydroxybenzoic acid--AMP ligase FadD22 (fadD22), partial CSB-YP450391MVS
CSB-EP450391MVS
CSB-BP450391MVS
CSB-MP450391MVS
CSB-EP450391MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Type III pantothenate kinase (coaX) CSB-YP450392MVS
CSB-EP450392MVS
CSB-BP450392MVS
CSB-MP450392MVS
CSB-EP450392MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 30S ribosomal protein S14 (rpsN) CSB-YP450393MVS
CSB-EP450393MVS
CSB-BP450393MVS
CSB-MP450393MVS
CSB-EP450393MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Thymidylate synthase thyX (thyX) CSB-YP450394MVS
CSB-EP450394MVS
CSB-BP450394MVS
CSB-MP450394MVS
CSB-EP450394MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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