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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brachypodium distachyon Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP451696BVS
CSB-EP451696BVS
CSB-BP451696BVS
CSB-MP451696BVS
CSB-EP451696BVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon NAD (P)H-quinone oxidoreductase subunit J, chloroplastic CSB-YP451697BVS
CSB-EP451697BVS
CSB-BP451697BVS
CSB-MP451697BVS
CSB-EP451697BVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP451698BVS
CSB-EP451698BVS
CSB-BP451698BVS
CSB-MP451698BVS
CSB-EP451698BVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon 30S ribosomal protein S15, chloroplastic (rps15-A) CSB-YP451699BVS
CSB-EP451699BVS
CSB-BP451699BVS
CSB-MP451699BVS
CSB-EP451699BVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic, partial CSB-YP451700BVS1
CSB-EP451700BVS1
CSB-BP451700BVS1
CSB-MP451700BVS1
CSB-EP451700BVS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana dybowskii Brevinin-1CDYa CSB-YP451701RJK
CSB-EP451701RJK
CSB-BP451701RJK
CSB-MP451701RJK
CSB-EP451701RJK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 30S ribosomal protein S6 (rpsF) CSB-YP451702LMJ
CSB-EP451702LMJ
CSB-BP451702LMJ
CSB-MP451702LMJ
CSB-EP451702LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Indole-3-glycerol phosphate synthase (trpC) CSB-YP451703LMJ
CSB-EP451703LMJ
CSB-BP451703LMJ
CSB-MP451703LMJ
CSB-EP451703LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Thiamine-phosphate synthase (thiE) CSB-YP451704LMJ
CSB-EP451704LMJ
CSB-BP451704LMJ
CSB-MP451704LMJ
CSB-EP451704LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei UPF0145 protein LCABL_07110 (LCABL_07110) CSB-YP451705LMJ
CSB-EP451705LMJ
CSB-BP451705LMJ
CSB-MP451705LMJ
CSB-EP451705LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 50S ribosomal protein L33 (rpmG) CSB-YP451706LMJ
CSB-EP451706LMJ
CSB-BP451706LMJ
CSB-MP451706LMJ
CSB-EP451706LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP451707LMJ
CSB-EP451707LMJ
CSB-BP451707LMJ
CSB-MP451707LMJ
CSB-EP451707LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei DNA mismatch repair protein MutS (mutS), partial CSB-YP451708LMJ
CSB-EP451708LMJ
CSB-BP451708LMJ
CSB-MP451708LMJ
CSB-EP451708LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei UPF0133 protein LCABL_24440 (LCABL_24440) CSB-YP451709LMJ
CSB-EP451709LMJ
CSB-BP451709LMJ
CSB-MP451709LMJ
CSB-EP451709LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 50S ribosomal protein L11 (rplK) CSB-YP451710LMJ
CSB-EP451710LMJ
CSB-BP451710LMJ
CSB-MP451710LMJ
CSB-EP451710LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 50S ribosomal protein L24 (rplX) CSB-YP451711LMJ
CSB-EP451711LMJ
CSB-BP451711LMJ
CSB-MP451711LMJ
CSB-EP451711LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 50S ribosomal protein L31 type B (rpmE2) CSB-YP451712LMJ
CSB-EP451712LMJ
CSB-BP451712LMJ
CSB-MP451712LMJ
CSB-EP451712LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei LexA repressor (lexA) CSB-YP451713LMJ
CSB-EP451713LMJ
CSB-BP451713LMJ
CSB-MP451713LMJ
CSB-EP451713LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Putative Holliday junction resolvase (LCABL_08480) CSB-YP451714LMJ
CSB-EP451714LMJ
CSB-BP451714LMJ
CSB-MP451714LMJ
CSB-EP451714LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei UPF0210 protein LCABL_10110 (LCABL_10110) CSB-YP451715LMJ
CSB-EP451715LMJ
CSB-BP451715LMJ
CSB-MP451715LMJ
CSB-EP451715LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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