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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Proteus mirabilis D-alanine--D-alanine ligase (ddl) CSB-YP451776EYZ
CSB-EP451776EYZ
CSB-BP451776EYZ
CSB-MP451776EYZ
CSB-EP451776EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis D-ribose pyranase (rbsD) CSB-YP451777EYZ
CSB-EP451777EYZ
CSB-BP451777EYZ
CSB-MP451777EYZ
CSB-EP451777EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Pantothenate synthetase (panC) CSB-YP451778EYZ
CSB-EP451778EYZ
CSB-BP451778EYZ
CSB-MP451778EYZ
CSB-EP451778EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase 1 (arnT1), partial CSB-YP451779EYZ
CSB-EP451779EYZ
CSB-BP451779EYZ
CSB-MP451779EYZ
CSB-EP451779EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Gamma-glutamyl phosphate reductase (proA) CSB-YP451780EYZ
CSB-EP451780EYZ
CSB-BP451780EYZ
CSB-MP451780EYZ
CSB-EP451780EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 30S ribosomal protein S16 (rpsP) CSB-YP451781EYZ
CSB-EP451781EYZ
CSB-BP451781EYZ
CSB-MP451781EYZ
CSB-EP451781EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP451782EYZ
CSB-EP451782EYZ
CSB-BP451782EYZ
CSB-MP451782EYZ
CSB-EP451782EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Outer-membrane lipoprotein carrier protein (lolA) CSB-YP451783EYZ
CSB-EP451783EYZ
CSB-BP451783EYZ
CSB-MP451783EYZ
CSB-EP451783EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ribosome modulation factor (rmf) CSB-YP451784EYZ
CSB-EP451784EYZ
CSB-BP451784EYZ
CSB-MP451784EYZ
CSB-EP451784EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis High frequency lysogenization protein HflD homolog (hflD) CSB-YP451785EYZ
CSB-EP451785EYZ
CSB-BP451785EYZ
CSB-MP451785EYZ
CSB-EP451785EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Probable septum site-determining protein MinC (minC) CSB-YP451786EYZ
CSB-EP451786EYZ
CSB-BP451786EYZ
CSB-MP451786EYZ
CSB-EP451786EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis GTP cyclohydrolase-2 (ribA) CSB-YP451787EYZ
CSB-EP451787EYZ
CSB-BP451787EYZ
CSB-MP451787EYZ
CSB-EP451787EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP451788EYZ
CSB-EP451788EYZ
CSB-BP451788EYZ
CSB-MP451788EYZ
CSB-EP451788EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP451789EYZ
CSB-EP451789EYZ
CSB-BP451789EYZ
CSB-MP451789EYZ
CSB-EP451789EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Fumarate reductase subunit C (frdC), partial CSB-YP451790EYZ1
CSB-EP451790EYZ1
CSB-BP451790EYZ1
CSB-MP451790EYZ1
CSB-EP451790EYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis UPF0442 protein PMI1443 (PMI1443), partial CSB-YP451791EYZ1
CSB-EP451791EYZ1
CSB-BP451791EYZ1
CSB-MP451791EYZ1
CSB-EP451791EYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L13 (rplM) CSB-YP451792EYZ
CSB-EP451792EYZ
CSB-BP451792EYZ
CSB-MP451792EYZ
CSB-EP451792EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Na (+)/H (+) antiporter nhaB, partial CSB-YP451793EYZ
CSB-EP451793EYZ
CSB-BP451793EYZ
CSB-MP451793EYZ
CSB-EP451793EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Non-canonical purine NTP phosphatase (PMI3715) CSB-YP451794EYZ
CSB-EP451794EYZ
CSB-BP451794EYZ
CSB-MP451794EYZ
CSB-EP451794EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Uracil phosphoribosyltransferase (upp) CSB-YP451795EYZ
CSB-EP451795EYZ
CSB-BP451795EYZ
CSB-MP451795EYZ
CSB-EP451795EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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