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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia phymatum 30S ribosomal protein S18 (rpsR) CSB-YP450576BXT
CSB-EP450576BXT
CSB-BP450576BXT
CSB-MP450576BXT
CSB-EP450576BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Protein grpE (grpE) CSB-YP450577BXT
CSB-EP450577BXT
CSB-BP450577BXT
CSB-MP450577BXT
CSB-EP450577BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Large-conductance mechanosensitive channel (mscL), partial CSB-YP450578BXT1
CSB-EP450578BXT1
CSB-BP450578BXT1
CSB-MP450578BXT1
CSB-EP450578BXT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP450579BXT
CSB-EP450579BXT
CSB-BP450579BXT
CSB-MP450579BXT
CSB-EP450579BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Proline--tRNA ligase (proS), partial CSB-YP450580BXT
CSB-EP450580BXT
CSB-BP450580BXT
CSB-MP450580BXT
CSB-EP450580BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L27 (rpmA) CSB-YP450581BXT
CSB-EP450581BXT
CSB-BP450581BXT
CSB-MP450581BXT
CSB-EP450581BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP450582BXT
CSB-EP450582BXT
CSB-BP450582BXT
CSB-MP450582BXT
CSB-EP450582BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 30S ribosomal protein S13 (rpsM) CSB-YP450583BXT
CSB-EP450583BXT
CSB-BP450583BXT
CSB-MP450583BXT
CSB-EP450583BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 30S ribosomal protein S8 (rpsH) CSB-YP450584BXT
CSB-EP450584BXT
CSB-BP450584BXT
CSB-MP450584BXT
CSB-EP450584BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 30S ribosomal protein S3 (rpsC) CSB-YP450585BXT
CSB-EP450585BXT
CSB-BP450585BXT
CSB-MP450585BXT
CSB-EP450585BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 50S ribosomal protein L23 (rplW) CSB-YP450586BXT
CSB-EP450586BXT
CSB-BP450586BXT
CSB-MP450586BXT
CSB-EP450586BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum DNA mismatch repair protein MutS (mutS), partial CSB-YP450587BXT
CSB-EP450587BXT
CSB-BP450587BXT
CSB-MP450587BXT
CSB-EP450587BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Histidine--tRNA ligase (hisS) CSB-YP450588BXT
CSB-EP450588BXT
CSB-BP450588BXT
CSB-MP450588BXT
CSB-EP450588BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Co-chaperone protein hscB homolog (hscB) CSB-YP450589BXT
CSB-EP450589BXT
CSB-BP450589BXT
CSB-MP450589BXT
CSB-EP450589BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Putative membrane protein insertion efficiency factor (Bphy_3099) CSB-YP450590BXT
CSB-EP450590BXT
CSB-BP450590BXT
CSB-MP450590BXT
CSB-EP450590BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP450591BXT
CSB-EP450591BXT
CSB-BP450591BXT
CSB-MP450591BXT
CSB-EP450591BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Chorismate synthase (aroC) CSB-YP450592BXT
CSB-EP450592BXT
CSB-BP450592BXT
CSB-MP450592BXT
CSB-EP450592BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum UPF0303 protein Bphy_1660 (Bphy_1660) CSB-YP450593BXT
CSB-EP450593BXT
CSB-BP450593BXT
CSB-MP450593BXT
CSB-EP450593BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum L-rhamnonate dehydratase (rhmD) CSB-YP450594BXT
CSB-EP450594BXT
CSB-BP450594BXT
CSB-MP450594BXT
CSB-EP450594BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum UPF0502 protein Bphy_5360 (Bphy_5360) CSB-YP450595BXT
CSB-EP450595BXT
CSB-BP450595BXT
CSB-MP450595BXT
CSB-EP450595BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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