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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella boydii serotype 18 Phosphoglucosamine mutase (glmM) CSB-YP450976SYZ
CSB-EP450976SYZ
CSB-BP450976SYZ
CSB-MP450976SYZ
CSB-EP450976SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Thr operon leader peptide (thrL) CSB-YP450977SYZ
CSB-EP450977SYZ
CSB-BP450977SYZ
CSB-MP450977SYZ
CSB-EP450977SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Glutathione-regulated potassium-efflux system protein kefC (kefC), partial CSB-YP450978SYZ
CSB-EP450978SYZ
CSB-BP450978SYZ
CSB-MP450978SYZ
CSB-EP450978SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP450979SYZ
CSB-EP450979SYZ
CSB-BP450979SYZ
CSB-MP450979SYZ
CSB-EP450979SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP450980SYZ
CSB-EP450980SYZ
CSB-BP450980SYZ
CSB-MP450980SYZ
CSB-EP450980SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Electron transport complex protein RnfD (rnfD), partial CSB-YP450981SYZ
CSB-EP450981SYZ
CSB-BP450981SYZ
CSB-MP450981SYZ
CSB-EP450981SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 HTH-type transcriptional repressor PurR (purR) CSB-YP450982SYZ
CSB-EP450982SYZ
CSB-BP450982SYZ
CSB-MP450982SYZ
CSB-EP450982SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0306 protein yhbP (yhbP) CSB-YP450983SYZ
CSB-EP450983SYZ
CSB-BP450983SYZ
CSB-MP450983SYZ
CSB-EP450983SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L18 (rplR) CSB-YP450984SYZ
CSB-EP450984SYZ
CSB-BP450984SYZ
CSB-MP450984SYZ
CSB-EP450984SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L29 (rpmC) CSB-YP450985SYZ
CSB-EP450985SYZ
CSB-BP450985SYZ
CSB-MP450985SYZ
CSB-EP450985SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 30S ribosomal protein S10 (rpsJ) CSB-YP450986SYZ
CSB-EP450986SYZ
CSB-BP450986SYZ
CSB-MP450986SYZ
CSB-EP450986SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0231 protein yacL (yacL) CSB-YP450987SYZ
CSB-EP450987SYZ
CSB-BP450987SYZ
CSB-MP450987SYZ
CSB-EP450987SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 H (+)/Cl (-) exchange transporter ClcA, partial CSB-YP450988SYZ
CSB-EP450988SYZ
CSB-BP450988SYZ
CSB-MP450988SYZ
CSB-EP450988SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Outer membrane protein assembly factor yaeT (yaeT), partial CSB-YP450989SYZ
CSB-EP450989SYZ
CSB-BP450989SYZ
CSB-MP450989SYZ
CSB-EP450989SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Ribonuclease H (rnhA) CSB-YP450990SYZ
CSB-EP450990SYZ
CSB-BP450990SYZ
CSB-MP450990SYZ
CSB-EP450990SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Glutathione-regulated potassium-efflux system protein KefB (kefB), partial CSB-YP450991SYZ
CSB-EP450991SYZ
CSB-BP450991SYZ
CSB-MP450991SYZ
CSB-EP450991SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Fe/S biogenesis protein NfuA (nfuA) CSB-YP450992SYZ
CSB-EP450992SYZ
CSB-BP450992SYZ
CSB-MP450992SYZ
CSB-EP450992SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 DNA polymerase IV (dinB) CSB-YP450993SYZ
CSB-EP450993SYZ
CSB-BP450993SYZ
CSB-MP450993SYZ
CSB-EP450993SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Glutamate 5-kinase (proB) CSB-YP450994SYZ
CSB-EP450994SYZ
CSB-BP450994SYZ
CSB-MP450994SYZ
CSB-EP450994SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Recombination-associated protein rdgC (rdgC) CSB-YP450995SYZ
CSB-EP450995SYZ
CSB-BP450995SYZ
CSB-MP450995SYZ
CSB-EP450995SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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