Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Nostoc punctiforme Proline--tRNA ligase (proS), partial CSB-YP450556NHQ
CSB-EP450556NHQ
CSB-BP450556NHQ
CSB-MP450556NHQ
CSB-EP450556NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP450557NHQ
CSB-EP450557NHQ
CSB-BP450557NHQ
CSB-MP450557NHQ
CSB-EP450557NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Probable transcriptional regulatory protein Npun_R5651 (Npun_R5651) CSB-YP450558NHQ
CSB-EP450558NHQ
CSB-BP450558NHQ
CSB-MP450558NHQ
CSB-EP450558NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Ribulose bisphosphate carboxylase large chain (cbbL) CSB-YP450559NHQ
CSB-EP450559NHQ
CSB-BP450559NHQ
CSB-MP450559NHQ
CSB-EP450559NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Adaptive-response sensory-kinase sasA (sasA) CSB-YP450560NHQ
CSB-EP450560NHQ
CSB-BP450560NHQ
CSB-MP450560NHQ
CSB-EP450560NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Protein psbN (psbN), partial CSB-YP450561NHQ
CSB-EP450561NHQ
CSB-BP450561NHQ
CSB-MP450561NHQ
CSB-EP450561NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 50S ribosomal protein L19 (rplS) CSB-YP450562NHQ
CSB-EP450562NHQ
CSB-BP450562NHQ
CSB-MP450562NHQ
CSB-EP450562NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Pyridoxamine kinase (pdxY) CSB-YP450563BXT
CSB-EP450563BXT
CSB-BP450563BXT
CSB-MP450563BXT
CSB-EP450563BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP450564BXT
CSB-EP450564BXT
CSB-BP450564BXT
CSB-MP450564BXT
CSB-EP450564BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP450565BXT1
CSB-EP450565BXT1
CSB-BP450565BXT1
CSB-MP450565BXT1
CSB-EP450565BXT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Glycerol kinase (glpK) CSB-YP450566BXT
CSB-EP450566BXT
CSB-BP450566BXT
CSB-MP450566BXT
CSB-EP450566BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Ketol-acid reductoisomerase (ilvC) CSB-YP450567BXT
CSB-EP450567BXT
CSB-BP450567BXT
CSB-MP450567BXT
CSB-EP450567BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum UPF0234 protein Bphy_0527 (Bphy_0527) CSB-YP450568BXT
CSB-EP450568BXT
CSB-BP450568BXT
CSB-MP450568BXT
CSB-EP450568BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 30S ribosomal protein S20 (rpsT) CSB-YP450569BXT
CSB-EP450569BXT
CSB-BP450569BXT
CSB-MP450569BXT
CSB-EP450569BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP450570BXT
CSB-EP450570BXT
CSB-BP450570BXT
CSB-MP450570BXT
CSB-EP450570BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Fructose-1,6-bisphosphatase class 1 1 (fbp1) CSB-YP450571BXT
CSB-EP450571BXT
CSB-BP450571BXT
CSB-MP450571BXT
CSB-EP450571BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Pantothenate synthetase (panC) CSB-YP450572BXT
CSB-EP450572BXT
CSB-BP450572BXT
CSB-MP450572BXT
CSB-EP450572BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Cytidylate kinase (cmk) CSB-YP450573BXT
CSB-EP450573BXT
CSB-BP450573BXT
CSB-MP450573BXT
CSB-EP450573BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Urease accessory protein UreG (ureG) CSB-YP450574BXT
CSB-EP450574BXT
CSB-BP450574BXT
CSB-MP450574BXT
CSB-EP450574BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Elongation factor 4 (lepA), partial CSB-YP450575BXT
CSB-EP450575BXT
CSB-BP450575BXT
CSB-MP450575BXT
CSB-EP450575BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>