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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella abortus Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP450756BWR
CSB-EP450756BWR
CSB-BP450756BWR
CSB-MP450756BWR
CSB-EP450756BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP450757BWR
CSB-EP450757BWR
CSB-BP450757BWR
CSB-MP450757BWR
CSB-EP450757BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Indole-3-glycerol phosphate synthase (trpC) CSB-YP450758BWR
CSB-EP450758BWR
CSB-BP450758BWR
CSB-MP450758BWR
CSB-EP450758BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP450759BWR
CSB-EP450759BWR
CSB-BP450759BWR
CSB-MP450759BWR
CSB-EP450759BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L17 (rplQ) CSB-YP450760BWR
CSB-EP450760BWR
CSB-BP450760BWR
CSB-MP450760BWR
CSB-EP450760BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 30S ribosomal protein S17 (rpsQ) CSB-YP450761BWR
CSB-EP450761BWR
CSB-BP450761BWR
CSB-MP450761BWR
CSB-EP450761BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L3 (rplC) CSB-YP450762BWR
CSB-EP450762BWR
CSB-BP450762BWR
CSB-MP450762BWR
CSB-EP450762BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L10 (rplJ) CSB-YP450763BWR
CSB-EP450763BWR
CSB-BP450763BWR
CSB-MP450763BWR
CSB-EP450763BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP450764BWR
CSB-EP450764BWR
CSB-BP450764BWR
CSB-MP450764BWR
CSB-EP450764BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP450765BWR
CSB-EP450765BWR
CSB-BP450765BWR
CSB-MP450765BWR
CSB-EP450765BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Malate synthase G (glcB), partial CSB-YP450766BWR
CSB-EP450766BWR
CSB-BP450766BWR
CSB-MP450766BWR
CSB-EP450766BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Elongation factor P (efp) CSB-YP450767BWR
CSB-EP450767BWR
CSB-BP450767BWR
CSB-MP450767BWR
CSB-EP450767BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP450768BWR
CSB-EP450768BWR
CSB-BP450768BWR
CSB-MP450768BWR
CSB-EP450768BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP450769BWR
CSB-EP450769BWR
CSB-BP450769BWR
CSB-MP450769BWR
CSB-EP450769BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Protein translocase subunit SecA (secA), partial CSB-YP450770BWR
CSB-EP450770BWR
CSB-BP450770BWR
CSB-MP450770BWR
CSB-EP450770BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP450771BWR
CSB-EP450771BWR
CSB-BP450771BWR
CSB-MP450771BWR
CSB-EP450771BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP450772BWR
CSB-EP450772BWR
CSB-BP450772BWR
CSB-MP450772BWR
CSB-EP450772BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 50S ribosomal protein L35 (rpmI) CSB-YP450773BWR
CSB-EP450773BWR
CSB-BP450773BWR
CSB-MP450773BWR
CSB-EP450773BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP450774BWR
CSB-EP450774BWR
CSB-BP450774BWR
CSB-MP450774BWR
CSB-EP450774BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus 30S ribosomal protein S20 (rpsT) CSB-YP450775BWR
CSB-EP450775BWR
CSB-BP450775BWR
CSB-MP450775BWR
CSB-EP450775BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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