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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Erwinia tasmaniensis Endonuclease V (nfi) CSB-YP451156ENB
CSB-EP451156ENB
CSB-BP451156ENB
CSB-MP451156ENB
CSB-EP451156ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP451157ENB
CSB-EP451157ENB
CSB-BP451157ENB
CSB-MP451157ENB
CSB-EP451157ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Glutamate racemase (murI) CSB-YP451158ENB
CSB-EP451158ENB
CSB-BP451158ENB
CSB-MP451158ENB
CSB-EP451158ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP451159ENB1
CSB-EP451159ENB1
CSB-BP451159ENB1
CSB-MP451159ENB1
CSB-EP451159ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Multifunctional CCA protein (cca) CSB-YP451160ENB
CSB-EP451160ENB
CSB-BP451160ENB
CSB-MP451160ENB
CSB-EP451160ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis RNA polymerase-associated protein RapA (rapA), partial CSB-YP451161ENB
CSB-EP451161ENB
CSB-BP451161ENB
CSB-MP451161ENB
CSB-EP451161ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Ribosome maturation factor RimP (rimP) CSB-YP451162ENB
CSB-EP451162ENB
CSB-BP451162ENB
CSB-MP451162ENB
CSB-EP451162ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 30S ribosomal protein S9 (rpsI) CSB-YP451163ENB
CSB-EP451163ENB
CSB-BP451163ENB
CSB-MP451163ENB
CSB-EP451163ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 3-dehydroquinate dehydratase (aroQ) CSB-YP451164ENB
CSB-EP451164ENB
CSB-BP451164ENB
CSB-MP451164ENB
CSB-EP451164ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Na (+)/H (+) antiporter nhaA, partial CSB-YP451165ENB
CSB-EP451165ENB
CSB-BP451165ENB
CSB-MP451165ENB
CSB-EP451165ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Glutamate--cysteine ligase (gshA), partial CSB-YP451166ENB
CSB-EP451166ENB
CSB-BP451166ENB
CSB-MP451166ENB
CSB-EP451166ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Universal stress protein B (uspB), partial CSB-YP451167ENB1
CSB-EP451167ENB1
CSB-BP451167ENB1
CSB-MP451167ENB1
CSB-EP451167ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis N utilization substance protein B homolog (nusB) CSB-YP451168ENB
CSB-EP451168ENB
CSB-BP451168ENB
CSB-MP451168ENB
CSB-EP451168ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Trigger factor (tig) CSB-YP451169ENB
CSB-EP451169ENB
CSB-BP451169ENB
CSB-MP451169ENB
CSB-EP451169ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP451170ENB
CSB-EP451170ENB
CSB-BP451170ENB
CSB-MP451170ENB
CSB-EP451170ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Elongation factor 4 (lepA), partial CSB-YP451171ENB
CSB-EP451171ENB
CSB-BP451171ENB
CSB-MP451171ENB
CSB-EP451171ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Methionine--tRNA ligase (metG), partial CSB-YP451172ENB
CSB-EP451172ENB
CSB-BP451172ENB
CSB-MP451172ENB
CSB-EP451172ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP451173ENB1
CSB-EP451173ENB1
CSB-BP451173ENB1
CSB-MP451173ENB1
CSB-EP451173ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP451174ENB
CSB-EP451174ENB
CSB-BP451174ENB
CSB-MP451174ENB
CSB-EP451174ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Cell division topological specificity factor (minE) CSB-YP451175ENB
CSB-EP451175ENB
CSB-BP451175ENB
CSB-MP451175ENB
CSB-EP451175ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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