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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ralstonia pickettii Probable acetoacetate decarboxylase (adc) CSB-YP451036RJI
CSB-EP451036RJI
CSB-BP451036RJI
CSB-MP451036RJI
CSB-EP451036RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP451037RJI
CSB-EP451037RJI
CSB-BP451037RJI
CSB-MP451037RJI
CSB-EP451037RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Alanine--tRNA ligase (alaS), partial CSB-YP451038AZF
CSB-EP451038AZF
CSB-BP451038AZF
CSB-MP451038AZF
CSB-EP451038AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 50S ribosomal protein L20 (rplT) CSB-YP451039AZF
CSB-EP451039AZF
CSB-BP451039AZF
CSB-MP451039AZF
CSB-EP451039AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Aspartate carbamoyltransferase (pyrB) CSB-YP451040AZF
CSB-EP451040AZF
CSB-BP451040AZF
CSB-MP451040AZF
CSB-EP451040AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP451041AZF1
CSB-EP451041AZF1
CSB-BP451041AZF1
CSB-MP451041AZF1
CSB-EP451041AZF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 30S ribosomal protein S7 (rpsG) CSB-YP451042AZF
CSB-EP451042AZF
CSB-BP451042AZF
CSB-MP451042AZF
CSB-EP451042AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila ATP-dependent zinc metalloprotease FtsH (ftsH), partial CSB-YP451043AZF
CSB-EP451043AZF
CSB-BP451043AZF
CSB-MP451043AZF
CSB-EP451043AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Pantothenate synthetase (panC) CSB-YP451044AZF
CSB-EP451044AZF
CSB-BP451044AZF
CSB-MP451044AZF
CSB-EP451044AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila ATP synthase gamma chain (atpG) CSB-YP451045AZF
CSB-EP451045AZF
CSB-BP451045AZF
CSB-MP451045AZF
CSB-EP451045AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP451046AZF
CSB-EP451046AZF
CSB-BP451046AZF
CSB-MP451046AZF
CSB-EP451046AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Probable transcriptional regulatory protein Amuc_0709 (Amuc_0709) CSB-YP451047AZF
CSB-EP451047AZF
CSB-BP451047AZF
CSB-MP451047AZF
CSB-EP451047AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Glycosyl hydrolase family 109 protein 2 (Amuc_0920) CSB-YP451048AZF
CSB-EP451048AZF
CSB-BP451048AZF
CSB-MP451048AZF
CSB-EP451048AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Type III pantothenate kinase (coaX) CSB-YP451049AZF
CSB-EP451049AZF
CSB-BP451049AZF
CSB-MP451049AZF
CSB-EP451049AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Trigger factor (tig) CSB-YP451050AZF
CSB-EP451050AZF
CSB-BP451050AZF
CSB-MP451050AZF
CSB-EP451050AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila UPF0133 protein Amuc_1227 (Amuc_1227) CSB-YP451051AZF
CSB-EP451051AZF
CSB-BP451051AZF
CSB-MP451051AZF
CSB-EP451051AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L35 (rpmI) CSB-YP451052HUZ
CSB-EP451052HUZ
CSB-BP451052HUZ
CSB-MP451052HUZ
CSB-EP451052HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Probable transcriptional regulatory protein HPSH_00820 (HPSH_00820) CSB-YP451053HUZ
CSB-EP451053HUZ
CSB-BP451053HUZ
CSB-MP451053HUZ
CSB-EP451053HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP451054HUZ
CSB-EP451054HUZ
CSB-BP451054HUZ
CSB-MP451054HUZ
CSB-EP451054HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L21 (rplU) CSB-YP451055HUZ
CSB-EP451055HUZ
CSB-BP451055HUZ
CSB-MP451055HUZ
CSB-EP451055HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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