Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium marinum 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP452377MVS
CSB-EP452377MVS
CSB-BP452377MVS
CSB-MP452377MVS
CSB-EP452377MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Demethylmenaquinone methyltransferase (ubiE) CSB-YP452378MVS
CSB-EP452378MVS
CSB-BP452378MVS
CSB-MP452378MVS
CSB-EP452378MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L11 (rplK) CSB-YP452379MVS
CSB-EP452379MVS
CSB-BP452379MVS
CSB-MP452379MVS
CSB-EP452379MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L2 (rplB) CSB-YP452380MVS
CSB-EP452380MVS
CSB-BP452380MVS
CSB-MP452380MVS
CSB-EP452380MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Urease subunit alpha (ureC), partial CSB-YP452381MVS
CSB-EP452381MVS
CSB-BP452381MVS
CSB-MP452381MVS
CSB-EP452381MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP452382AWO
CSB-EP452382AWO
CSB-BP452382AWO
CSB-MP452382AWO
CSB-EP452382AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP452383AWO
CSB-EP452383AWO
CSB-BP452383AWO
CSB-MP452383AWO
CSB-EP452383AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 30S ribosomal protein S12 (rpsL) CSB-YP452384AWO
CSB-EP452384AWO
CSB-BP452384AWO
CSB-MP452384AWO
CSB-EP452384AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Putative arginyl-tRNA--protein transferase (ate) CSB-YP452385AWO
CSB-EP452385AWO
CSB-BP452385AWO
CSB-MP452385AWO
CSB-EP452385AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP452386AWO
CSB-EP452386AWO
CSB-BP452386AWO
CSB-MP452386AWO
CSB-EP452386AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP452387AWO
CSB-EP452387AWO
CSB-BP452387AWO
CSB-MP452387AWO
CSB-EP452387AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Indole-3-glycerol phosphate synthase (trpC) CSB-YP452388AWO
CSB-EP452388AWO
CSB-BP452388AWO
CSB-MP452388AWO
CSB-EP452388AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Urease accessory protein UreD (ureD) CSB-YP452389AWO
CSB-EP452389AWO
CSB-BP452389AWO
CSB-MP452389AWO
CSB-EP452389AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Carbon storage regulator homolog (csrA) CSB-YP452390AWO
CSB-EP452390AWO
CSB-BP452390AWO
CSB-MP452390AWO
CSB-EP452390AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP452391AWO
CSB-EP452391AWO
CSB-BP452391AWO
CSB-MP452391AWO
CSB-EP452391AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Protease HtpX (htpX), partial CSB-YP452392AWO1
CSB-EP452392AWO1
CSB-BP452392AWO1
CSB-MP452392AWO1
CSB-EP452392AWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L27 (rpmA) CSB-YP452393AWO
CSB-EP452393AWO
CSB-BP452393AWO
CSB-MP452393AWO
CSB-EP452393AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP452394AWO
CSB-EP452394AWO
CSB-BP452394AWO
CSB-MP452394AWO
CSB-EP452394AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP452395AWO
CSB-EP452395AWO
CSB-BP452395AWO
CSB-MP452395AWO
CSB-EP452395AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii S-adenosylmethionine synthase (metK) CSB-YP452396AWO
CSB-EP452396AWO
CSB-BP452396AWO
CSB-MP452396AWO
CSB-EP452396AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>