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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella boydii serotype 18 Regulator of sigma D (rsd) CSB-YP452897SYZ
CSB-EP452897SYZ
CSB-BP452897SYZ
CSB-MP452897SYZ
CSB-EP452897SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Molecular chaperone Hsp31 and glyoxalase 3 (hchA) CSB-YP452898SYZ
CSB-EP452898SYZ
CSB-BP452898SYZ
CSB-MP452898SYZ
CSB-EP452898SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP452899SYZ
CSB-EP452899SYZ
CSB-BP452899SYZ
CSB-MP452899SYZ
CSB-EP452899SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 HTH-type transcriptional regulator murR (murR) CSB-YP452900SYZ
CSB-EP452900SYZ
CSB-BP452900SYZ
CSB-MP452900SYZ
CSB-EP452900SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP452901SYZ1
CSB-EP452901SYZ1
CSB-BP452901SYZ1
CSB-MP452901SYZ1
CSB-EP452901SYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Dihydrodipicolinate synthase (dapA) CSB-YP452902SYZ
CSB-EP452902SYZ
CSB-BP452902SYZ
CSB-MP452902SYZ
CSB-EP452902SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Histidine--tRNA ligase (hisS) CSB-YP452903SYZ
CSB-EP452903SYZ
CSB-BP452903SYZ
CSB-MP452903SYZ
CSB-EP452903SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 60 kDa chaperonin (groL), partial CSB-YP452904SYZ
CSB-EP452904SYZ
CSB-BP452904SYZ
CSB-MP452904SYZ
CSB-EP452904SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 HTH-type transcriptional regulator UlaR (ulaR) CSB-YP452905SYZ
CSB-EP452905SYZ
CSB-BP452905SYZ
CSB-MP452905SYZ
CSB-EP452905SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Multidrug resistance protein MdtA (mdtA) CSB-YP452906SYZ
CSB-EP452906SYZ
CSB-BP452906SYZ
CSB-MP452906SYZ
CSB-EP452906SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 DNA gyrase inhibitor (sbmC) CSB-YP452907SYZ
CSB-EP452907SYZ
CSB-BP452907SYZ
CSB-MP452907SYZ
CSB-EP452907SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L19 (rplS) CSB-YP452908SYZ
CSB-EP452908SYZ
CSB-BP452908SYZ
CSB-MP452908SYZ
CSB-EP452908SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 DNA mismatch repair protein mutH (mutH) CSB-YP452909SYZ
CSB-EP452909SYZ
CSB-BP452909SYZ
CSB-MP452909SYZ
CSB-EP452909SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Probable cytosol aminopeptidase (pepA), partial CSB-YP452910SYZ
CSB-EP452910SYZ
CSB-BP452910SYZ
CSB-MP452910SYZ
CSB-EP452910SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Cell division topological specificity factor (minE) CSB-YP452911SYZ
CSB-EP452911SYZ
CSB-BP452911SYZ
CSB-MP452911SYZ
CSB-EP452911SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP452912SYZ
CSB-EP452912SYZ
CSB-BP452912SYZ
CSB-MP452912SYZ
CSB-EP452912SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Adenylyl-sulfate kinase (cysC) CSB-YP452913SYZ
CSB-EP452913SYZ
CSB-BP452913SYZ
CSB-MP452913SYZ
CSB-EP452913SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP452914SYZ
CSB-EP452914SYZ
CSB-BP452914SYZ
CSB-MP452914SYZ
CSB-EP452914SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP452915SYZ
CSB-EP452915SYZ
CSB-BP452915SYZ
CSB-MP452915SYZ
CSB-EP452915SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Lipoate-protein ligase A (lplA) CSB-YP452916SYZ
CSB-EP452916SYZ
CSB-BP452916SYZ
CSB-MP452916SYZ
CSB-EP452916SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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