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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia cepacia NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP451736BXS
CSB-EP451736BXS
CSB-BP451736BXS
CSB-MP451736BXS
CSB-EP451736BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP451737BXS
CSB-EP451737BXS
CSB-BP451737BXS
CSB-MP451737BXS
CSB-EP451737BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Uracil phosphoribosyltransferase (upp) CSB-YP451738BXS
CSB-EP451738BXS
CSB-BP451738BXS
CSB-MP451738BXS
CSB-EP451738BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP451739BXS
CSB-EP451739BXS
CSB-BP451739BXS
CSB-MP451739BXS
CSB-EP451739BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC) CSB-YP451740BXS
CSB-BP451740BXS
CSB-MP451740BXS
CSB-EP451740BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Protein CyaY (cyaY) CSB-YP451741BXS
CSB-EP451741BXS
CSB-BP451741BXS
CSB-MP451741BXS
CSB-EP451741BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP451742BXS
CSB-EP451742BXS
CSB-BP451742BXS
CSB-MP451742BXS
CSB-EP451742BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Protein MraZ (mraZ) CSB-YP451743BXS
CSB-EP451743BXS
CSB-BP451743BXS
CSB-MP451743BXS
CSB-EP451743BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia 50S ribosomal protein L35 (rpmI) CSB-YP451744BXS
CSB-EP451744BXS
CSB-BP451744BXS
CSB-MP451744BXS
CSB-EP451744BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Ribosome maturation factor RimP (rimP) CSB-YP451745BXS
CSB-EP451745BXS
CSB-BP451745BXS
CSB-MP451745BXS
CSB-EP451745BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP451746BXS
CSB-EP451746BXS
CSB-BP451746BXS
CSB-MP451746BXS
CSB-EP451746BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Flagellar L-ring protein (flgH) CSB-YP451747BXS
CSB-EP451747BXS
CSB-BP451747BXS
CSB-MP451747BXS
CSB-EP451747BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP451748BXS
CSB-EP451748BXS
CSB-BP451748BXS
CSB-MP451748BXS
CSB-EP451748BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP451749BXS
CSB-EP451749BXS
CSB-BP451749BXS
CSB-MP451749BXS
CSB-EP451749BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Biotin synthase (bioB) CSB-YP451750BXS
CSB-EP451750BXS
CSB-BP451750BXS
CSB-MP451750BXS
CSB-EP451750BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP451751BXS
CSB-EP451751BXS
CSB-BP451751BXS
CSB-MP451751BXS
CSB-EP451751BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia UPF0307 protein BceJ2315_28530 (BceJ2315_28530) CSB-YP451752BXS
CSB-EP451752BXS
CSB-BP451752BXS
CSB-MP451752BXS
CSB-EP451752BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Protein hfq (hfq) CSB-YP451753BXS
CSB-EP451753BXS
CSB-BP451753BXS
CSB-MP451753BXS
CSB-EP451753BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Histidine--tRNA ligase (hisS) CSB-YP451754BXS
CSB-EP451754BXS
CSB-BP451754BXS
CSB-MP451754BXS
CSB-EP451754BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP451755BXS
CSB-EP451755BXS
CSB-BP451755BXS
CSB-MP451755BXS
CSB-EP451755BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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