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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chlorobium limicola UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP453297DSR
CSB-EP453297DSR
CSB-BP453297DSR
CSB-MP453297DSR
CSB-EP453297DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Shikimate kinase (aroK) CSB-YP453298DSR
CSB-EP453298DSR
CSB-BP453298DSR
CSB-MP453298DSR
CSB-EP453298DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Arginine repressor (argR) CSB-YP453299DSS
CSB-EP453299DSS
CSB-BP453299DSS
CSB-MP453299DSS
CSB-EP453299DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides DNA replication and repair protein recF (recF) CSB-YP453300DSS
CSB-EP453300DSS
CSB-BP453300DSS
CSB-MP453300DSS
CSB-EP453300DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP453301DSS
CSB-EP453301DSS
CSB-BP453301DSS
CSB-MP453301DSS
CSB-EP453301DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L9 (rplI) CSB-YP453302DSS
CSB-EP453302DSS
CSB-BP453302DSS
CSB-MP453302DSS
CSB-EP453302DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP453303DSS
CSB-EP453303DSS
CSB-BP453303DSS
CSB-MP453303DSS
CSB-EP453303DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP453304DSS
CSB-EP453304DSS
CSB-BP453304DSS
CSB-MP453304DSS
CSB-EP453304DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides CinA-like protein (Cphamn1_0300) CSB-YP453305DSS
CSB-EP453305DSS
CSB-BP453305DSS
CSB-MP453305DSS
CSB-EP453305DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP453306DSS
CSB-EP453306DSS
CSB-BP453306DSS
CSB-MP453306DSS
CSB-EP453306DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Probable pyruvoyl-dependent arginine decarboxylase (pdaD) CSB-YP453307DSS
CSB-EP453307DSS
CSB-BP453307DSS
CSB-MP453307DSS
CSB-EP453307DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP453308DSS
CSB-EP453308DSS
CSB-BP453308DSS
CSB-MP453308DSS
CSB-EP453308DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Regulatory protein recX (recX) CSB-YP453309DSS
CSB-EP453309DSS
CSB-BP453309DSS
CSB-MP453309DSS
CSB-EP453309DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Leucine--tRNA ligase (leuS), partial CSB-YP453310DSS
CSB-EP453310DSS
CSB-BP453310DSS
CSB-MP453310DSS
CSB-EP453310DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Pantothenate synthetase (panC) CSB-YP453311DSS
CSB-EP453311DSS
CSB-BP453311DSS
CSB-MP453311DSS
CSB-EP453311DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L28 (rpmB) CSB-YP453312DSS
CSB-EP453312DSS
CSB-BP453312DSS
CSB-MP453312DSS
CSB-EP453312DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides UPF0235 protein Cphamn1_2066 (Cphamn1_2066) CSB-YP453313DSS
CSB-EP453313DSS
CSB-BP453313DSS
CSB-MP453313DSS
CSB-EP453313DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Trigger factor (tig) CSB-YP453314DSS
CSB-EP453314DSS
CSB-BP453314DSS
CSB-MP453314DSS
CSB-EP453314DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 60 kDa chaperonin (groL), partial CSB-YP453315DSS
CSB-EP453315DSS
CSB-BP453315DSS
CSB-MP453315DSS
CSB-EP453315DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L27 (rpmA) CSB-YP453316DSS
CSB-EP453316DSS
CSB-BP453316DSS
CSB-MP453316DSS
CSB-EP453316DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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