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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella boydii serotype 18 50S ribosomal protein L4 (rplD) CSB-YP452937SYZ
CSB-EP452937SYZ
CSB-BP452937SYZ
CSB-MP452937SYZ
CSB-EP452937SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Protein slyX (slyX) CSB-YP452938SYZ
CSB-EP452938SYZ
CSB-BP452938SYZ
CSB-MP452938SYZ
CSB-EP452938SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP452939SYZ
CSB-EP452939SYZ
CSB-BP452939SYZ
CSB-MP452939SYZ
CSB-EP452939SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP452940SYZ
CSB-EP452940SYZ
CSB-BP452940SYZ
CSB-MP452940SYZ
CSB-EP452940SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP452941SYZ
CSB-EP452941SYZ
CSB-BP452941SYZ
CSB-MP452941SYZ
CSB-EP452941SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Ribonuclease HII (rnhB) CSB-YP452942SYZ
CSB-EP452942SYZ
CSB-BP452942SYZ
CSB-MP452942SYZ
CSB-EP452942SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L35 (rpmI) CSB-YP452943SYZ
CSB-EP452943SYZ
CSB-BP452943SYZ
CSB-MP452943SYZ
CSB-EP452943SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Arginine N-succinyltransferase (astA) CSB-YP452944SYZ
CSB-EP452944SYZ
CSB-BP452944SYZ
CSB-MP452944SYZ
CSB-EP452944SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Thiosulfate sulfurtransferase glpE (glpE) CSB-YP452945SYZ
CSB-EP452945SYZ
CSB-BP452945SYZ
CSB-MP452945SYZ
CSB-EP452945SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP452946SYZ
CSB-EP452946SYZ
CSB-BP452946SYZ
CSB-MP452946SYZ
CSB-EP452946SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Esterase FrsA (frsA) CSB-YP452947SYZ
CSB-EP452947SYZ
CSB-BP452947SYZ
CSB-MP452947SYZ
CSB-EP452947SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Anti-adapter protein iraP (iraP) CSB-YP452948SYZ
CSB-EP452948SYZ
CSB-BP452948SYZ
CSB-MP452948SYZ
CSB-EP452948SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0229 protein yeaH (yeaH) CSB-YP452949SYZ
CSB-EP452949SYZ
CSB-BP452949SYZ
CSB-MP452949SYZ
CSB-EP452949SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 ProP effector (proQ) CSB-YP452950SYZ
CSB-EP452950SYZ
CSB-BP452950SYZ
CSB-MP452950SYZ
CSB-EP452950SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Glycogen synthase (glgA) CSB-YP452951SYZ
CSB-EP452951SYZ
CSB-BP452951SYZ
CSB-MP452951SYZ
CSB-EP452951SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 7-cyano-7-deazaguanine synthase (queC) CSB-YP452952SYZ
CSB-EP452952SYZ
CSB-BP452952SYZ
CSB-MP452952SYZ
CSB-EP452952SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Adenylate kinase (adk) CSB-YP452953SYZ
CSB-EP452953SYZ
CSB-BP452953SYZ
CSB-MP452953SYZ
CSB-EP452953SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0227 protein ycfP (ycfP) CSB-YP452954SYZ
CSB-EP452954SYZ
CSB-BP452954SYZ
CSB-MP452954SYZ
CSB-EP452954SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP452955SYZ
CSB-EP452955SYZ
CSB-BP452955SYZ
CSB-MP452955SYZ
CSB-EP452955SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Urease subunit gamma (ureA) CSB-YP452956RJI
CSB-EP452956RJI
CSB-BP452956RJI
CSB-MP452956RJI
CSB-EP452956RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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