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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 7 RNA pyrophosphohydrolase (rppH) CSB-YP453377AXI
CSB-EP453377AXI
CSB-BP453377AXI
CSB-MP453377AXI
CSB-EP453377AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Met repressor (metJ) CSB-YP453378AXI
CSB-EP453378AXI
CSB-BP453378AXI
CSB-MP453378AXI
CSB-EP453378AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L34 (rpmH) CSB-YP453379AXI
CSB-EP453379AXI
CSB-BP453379AXI
CSB-MP453379AXI
CSB-EP453379AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP453380AXI
CSB-EP453380AXI
CSB-BP453380AXI
CSB-MP453380AXI
CSB-EP453380AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L33 (rpmG) CSB-YP453381AXI
CSB-EP453381AXI
CSB-BP453381AXI
CSB-MP453381AXI
CSB-EP453381AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative small ubiquitin-related modifier 8 (SUMO8) CSB-YP453382DOA
CSB-EP453382DOA
CSB-BP453382DOA
CSB-MP453382DOA
CSB-EP453382DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcription factor STP1 (STP1), partial CSB-YP453383SVP
CSB-EP453383SVP
CSB-BP453383SVP
CSB-MP453383SVP
CSB-EP453383SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Bis (5'-adenosyl)-triphosphatase CSB-YP453384SVP
CSB-EP453384SVP
CSB-BP453384SVP
CSB-MP453384SVP
CSB-EP453384SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Monopolar spindle protein 2 (MPS2), partial CSB-YP453385SVP1
CSB-EP453385SVP1
CSB-BP453385SVP1
CSB-MP453385SVP1
CSB-EP453385SVP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Succinate dehydrogenase assembly factor 2, mitochondrial (EMI5) CSB-YP453386SVP
CSB-EP453386SVP
CSB-BP453386SVP
CSB-MP453386SVP
CSB-EP453386SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 41, mitochondrial (AIM41) CSB-YP453387SVP
CSB-EP453387SVP
CSB-BP453387SVP
CSB-MP453387SVP
CSB-EP453387SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ASTRA-associated protein 1 (ASA1) CSB-YP453388SVP
CSB-EP453388SVP
CSB-BP453388SVP
CSB-MP453388SVP
CSB-EP453388SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 43, mitochondrial (AIM43), partial CSB-YP453389SVP1
CSB-EP453389SVP1
CSB-BP453389SVP1
CSB-MP453389SVP1
CSB-EP453389SVP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 34, mitochondrial (AIM34), partial CSB-YP453390SVP1
CSB-EP453390SVP1
CSB-BP453390SVP1
CSB-MP453390SVP1
CSB-EP453390SVP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (SCRG_01975), partial CSB-YP453391SVP
CSB-EP453391SVP
CSB-BP453391SVP
CSB-MP453391SVP
CSB-EP453391SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Acetyl-CoA carboxylase, mitochondrial (HFA1), partial CSB-YP453392SVP
CSB-EP453392SVP
CSB-BP453392SVP
CSB-MP453392SVP
CSB-EP453392SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Probable zinc metalloprotease SCRG_02892 (SCRG_02892), partial CSB-YP453393SVP
CSB-EP453393SVP
CSB-BP453393SVP
CSB-MP453393SVP
CSB-EP453393SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein SWT21 (SWT21) CSB-YP453394SVP
CSB-EP453394SVP
CSB-BP453394SVP
CSB-MP453394SVP
CSB-EP453394SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cysteine proteinase 1, mitochondrial (LAP3) CSB-YP453395SVP
CSB-EP453395SVP
CSB-BP453395SVP
CSB-MP453395SVP
CSB-EP453395SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cell wall mannoprotein HSP150 (HSP150) CSB-YP453396SVP
CSB-EP453396SVP
CSB-BP453396SVP
CSB-MP453396SVP
CSB-EP453396SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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