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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chlorobium phaeobacteroides Dihydrodipicolinate reductase (dapB) CSB-YP455278DSS
CSB-EP455278DSS
CSB-BP455278DSS
CSB-MP455278DSS
CSB-EP455278DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP455279DSS
CSB-EP455279DSS
CSB-BP455279DSS
CSB-MP455279DSS
CSB-EP455279DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Tryptophan synthase alpha chain (trpA) CSB-YP455280DSS
CSB-EP455280DSS
CSB-BP455280DSS
CSB-MP455280DSS
CSB-EP455280DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP455281DSS
CSB-EP455281DSS
CSB-BP455281DSS
CSB-MP455281DSS
CSB-EP455281DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 30S ribosomal protein S13 (rpsM) CSB-YP455282DSS
CSB-EP455282DSS
CSB-BP455282DSS
CSB-MP455282DSS
CSB-EP455282DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L16 (rplP) CSB-YP455283DSS
CSB-EP455283DSS
CSB-BP455283DSS
CSB-MP455283DSS
CSB-EP455283DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 30S ribosomal protein S19 (rpsS) CSB-YP455284DSS
CSB-EP455284DSS
CSB-BP455284DSS
CSB-MP455284DSS
CSB-EP455284DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 30S ribosomal protein S12 (rpsL) CSB-YP455285DSS
CSB-EP455285DSS
CSB-BP455285DSS
CSB-MP455285DSS
CSB-EP455285DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Elongation factor 4 (lepA), partial CSB-YP455286DSS
CSB-EP455286DSS
CSB-BP455286DSS
CSB-MP455286DSS
CSB-EP455286DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Putative Holliday junction resolvase (Cphamn1_2429) CSB-YP455287DSS
CSB-EP455287DSS
CSB-BP455287DSS
CSB-MP455287DSS
CSB-EP455287DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP455288DSS
CSB-EP455288DSS
CSB-BP455288DSS
CSB-MP455288DSS
CSB-EP455288DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Membrane protein insertase YidC (yidC), partial CSB-YP455289DSS1
CSB-EP455289DSS1
CSB-BP455289DSS1
CSB-MP455289DSS1
CSB-EP455289DSS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus Membrane protein insertase YidC (yidC), partial CSB-YP455290BYI1
CSB-EP455290BYI1
CSB-BP455290BYI1
CSB-MP455290BYI1
CSB-EP455290BYI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP455291BYI
CSB-EP455291BYI
CSB-BP455291BYI
CSB-MP455291BYI
CSB-EP455291BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus 50S ribosomal protein L35 (rpmI) CSB-YP455292BYI
CSB-EP455292BYI
CSB-BP455292BYI
CSB-MP455292BYI
CSB-EP455292BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP455293BYI
CSB-EP455293BYI
CSB-BP455293BYI
CSB-MP455293BYI
CSB-EP455293BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP455294BYI
CSB-EP455294BYI
CSB-BP455294BYI
CSB-MP455294BYI
CSB-EP455294BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus 50S ribosomal protein L19 (rplS) CSB-YP455295BYI
CSB-EP455295BYI
CSB-BP455295BYI
CSB-MP455295BYI
CSB-EP455295BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus Leucine--tRNA ligase (leuS) CSB-YP455296BYI
CSB-EP455296BYI
CSB-BP455296BYI
CSB-MP455296BYI
CSB-EP455296BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amoebophilus asiaticus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP455297BYI
CSB-EP455297BYI
CSB-BP455297BYI
CSB-MP455297BYI
CSB-EP455297BYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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