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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chlorobium limicola Dihydrodipicolinate reductase (dapB) CSB-YP455258DSR
CSB-EP455258DSR
CSB-BP455258DSR
CSB-MP455258DSR
CSB-EP455258DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP455259DSR
CSB-EP455259DSR
CSB-BP455259DSR
CSB-MP455259DSR
CSB-EP455259DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 30S ribosomal protein S4 (rpsD) CSB-YP455260DSR
CSB-EP455260DSR
CSB-BP455260DSR
CSB-MP455260DSR
CSB-EP455260DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 50S ribosomal protein L18 (rplR) CSB-YP455261DSR
CSB-EP455261DSR
CSB-BP455261DSR
CSB-MP455261DSR
CSB-EP455261DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 50S ribosomal protein L3 (rplC) CSB-YP455262DSR
CSB-EP455262DSR
CSB-BP455262DSR
CSB-MP455262DSR
CSB-EP455262DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Thiazole synthase (thiG) CSB-YP455263DSR
CSB-EP455263DSR
CSB-BP455263DSR
CSB-MP455263DSR
CSB-EP455263DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP455264DSR
CSB-EP455264DSR
CSB-BP455264DSR
CSB-MP455264DSR
CSB-EP455264DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola DNA repair protein recO (recO) CSB-YP455265DSR
CSB-EP455265DSR
CSB-BP455265DSR
CSB-MP455265DSR
CSB-EP455265DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP455266DSR
CSB-EP455266DSR
CSB-BP455266DSR
CSB-MP455266DSR
CSB-EP455266DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP455267DSR
CSB-EP455267DSR
CSB-BP455267DSR
CSB-MP455267DSR
CSB-EP455267DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola 50S ribosomal protein L34 (rpmH) CSB-YP455268DSR
CSB-EP455268DSR
CSB-BP455268DSR
CSB-MP455268DSR
CSB-EP455268DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Adenylate kinase (adk) CSB-YP455269DSS
CSB-EP455269DSS
CSB-BP455269DSS
CSB-MP455269DSS
CSB-EP455269DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP455270DSS
CSB-EP455270DSS
CSB-BP455270DSS
CSB-MP455270DSS
CSB-EP455270DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP455271DSS
CSB-EP455271DSS
CSB-BP455271DSS
CSB-MP455271DSS
CSB-EP455271DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Homoserine kinase (thrB) CSB-YP455272DSS
CSB-EP455272DSS
CSB-BP455272DSS
CSB-MP455272DSS
CSB-EP455272DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L7/L12 (rplL) CSB-YP455273DSS
CSB-EP455273DSS
CSB-BP455273DSS
CSB-MP455273DSS
CSB-EP455273DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Shikimate dehydrogenase (aroE) CSB-YP455274DSS
CSB-EP455274DSS
CSB-BP455274DSS
CSB-MP455274DSS
CSB-EP455274DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP455275DSS
CSB-EP455275DSS
CSB-BP455275DSS
CSB-MP455275DSS
CSB-EP455275DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP455276DSS
CSB-EP455276DSS
CSB-BP455276DSS
CSB-MP455276DSS
CSB-EP455276DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP455277DSS
CSB-EP455277DSS
CSB-BP455277DSS
CSB-MP455277DSS
CSB-EP455277DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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