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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Proteus mirabilis Cell division topological specificity factor (minE) CSB-YP455679EYZ
CSB-EP455679EYZ
CSB-BP455679EYZ
CSB-MP455679EYZ
CSB-EP455679EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Bifunctional protein HldE (hldE) CSB-YP455680EYZ
CSB-EP455680EYZ
CSB-BP455680EYZ
CSB-MP455680EYZ
CSB-EP455680EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP455681EYZ
CSB-EP455681EYZ
CSB-BP455681EYZ
CSB-MP455681EYZ
CSB-EP455681EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Sec-independent protein translocase protein TatA (tatA), partial CSB-YP455682EYZ
CSB-EP455682EYZ
CSB-BP455682EYZ
CSB-MP455682EYZ
CSB-EP455682EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis UPF0283 membrane protein PMI1371 (PMI1371), partial CSB-YP455683EYZ1
CSB-EP455683EYZ1
CSB-BP455683EYZ1
CSB-MP455683EYZ1
CSB-EP455683EYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis DNA-binding protein fis (fis) CSB-YP455684EYZ
CSB-EP455684EYZ
CSB-BP455684EYZ
CSB-MP455684EYZ
CSB-EP455684EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 60 kDa chaperonin (groL), partial CSB-YP455685EYZ
CSB-EP455685EYZ
CSB-BP455685EYZ
CSB-MP455685EYZ
CSB-EP455685EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Crotonobetainyl-CoA dehydrogenase (caiA) CSB-YP455686EYZ
CSB-EP455686EYZ
CSB-BP455686EYZ
CSB-MP455686EYZ
CSB-EP455686EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Multidrug resistance protein MdtA (mdtA) CSB-YP455687EYZ
CSB-EP455687EYZ
CSB-BP455687EYZ
CSB-MP455687EYZ
CSB-EP455687EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP455688EYZ
CSB-EP455688EYZ
CSB-BP455688EYZ
CSB-MP455688EYZ
CSB-EP455688EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Thiamine-phosphate synthase (thiE) CSB-YP455689EYZ
CSB-EP455689EYZ
CSB-BP455689EYZ
CSB-MP455689EYZ
CSB-EP455689EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L11 (rplK) CSB-YP455690EYZ
CSB-EP455690EYZ
CSB-BP455690EYZ
CSB-MP455690EYZ
CSB-EP455690EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis o-succinylbenzoate synthase (menC) CSB-YP455691EYZ
CSB-EP455691EYZ
CSB-BP455691EYZ
CSB-MP455691EYZ
CSB-EP455691EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP455692EYZ
CSB-EP455692EYZ
CSB-BP455692EYZ
CSB-MP455692EYZ
CSB-EP455692EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis DNA ligase (ligA), partial CSB-YP455693EYZ
CSB-EP455693EYZ
CSB-BP455693EYZ
CSB-MP455693EYZ
CSB-EP455693EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Co-chaperone protein hscB (hscB) CSB-YP455694EYZ
CSB-EP455694EYZ
CSB-BP455694EYZ
CSB-MP455694EYZ
CSB-EP455694EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Protein RnfH (rnfH) CSB-YP455695EYZ
CSB-EP455695EYZ
CSB-BP455695EYZ
CSB-MP455695EYZ
CSB-EP455695EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis ATP synthase gamma chain (atpG) CSB-YP455696EYZ
CSB-EP455696EYZ
CSB-BP455696EYZ
CSB-MP455696EYZ
CSB-EP455696EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis L-seryl-tRNA (Sec) selenium transferase CSB-YP455697EYZ
CSB-EP455697EYZ
CSB-BP455697EYZ
CSB-MP455697EYZ
CSB-EP455697EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ribonuclease P protein component (rnpA) CSB-YP455698EYZ
CSB-EP455698EYZ
CSB-BP455698EYZ
CSB-MP455698EYZ
CSB-EP455698EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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