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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Proteus mirabilis UPF0231 protein PMI2039 (PMI2039) CSB-YP455699EYZ
CSB-EP455699EYZ
CSB-BP455699EYZ
CSB-MP455699EYZ
CSB-EP455699EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Met repressor (metJ) CSB-YP455700EYZ
CSB-EP455700EYZ
CSB-BP455700EYZ
CSB-MP455700EYZ
CSB-EP455700EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP455701EYZ
CSB-EP455701EYZ
CSB-BP455701EYZ
CSB-MP455701EYZ
CSB-EP455701EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L2 (rplB) CSB-YP455702EYZ
CSB-EP455702EYZ
CSB-BP455702EYZ
CSB-MP455702EYZ
CSB-EP455702EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L24 (rplX) CSB-YP455703EYZ
CSB-EP455703EYZ
CSB-BP455703EYZ
CSB-MP455703EYZ
CSB-EP455703EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L15 (rplO) CSB-YP455704EYZ
CSB-EP455704EYZ
CSB-BP455704EYZ
CSB-MP455704EYZ
CSB-EP455704EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Large-conductance mechanosensitive channel (mscL), partial CSB-YP455705EYZ1
CSB-EP455705EYZ1
CSB-BP455705EYZ1
CSB-MP455705EYZ1
CSB-EP455705EYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP455706EYZ
CSB-EP455706EYZ
CSB-BP455706EYZ
CSB-MP455706EYZ
CSB-EP455706EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ketol-acid reductoisomerase (ilvC) CSB-YP455707EYZ
CSB-EP455707EYZ
CSB-BP455707EYZ
CSB-MP455707EYZ
CSB-EP455707EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP455708EYZ
CSB-EP455708EYZ
CSB-BP455708EYZ
CSB-MP455708EYZ
CSB-EP455708EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP455709EYZ
CSB-EP455709EYZ
CSB-BP455709EYZ
CSB-MP455709EYZ
CSB-EP455709EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 30S ribosomal protein S18 (rpsR) CSB-YP455710EYZ
CSB-EP455710EYZ
CSB-BP455710EYZ
CSB-MP455710EYZ
CSB-EP455710EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ribosome maturation factor RimP (rimP) CSB-YP455711EYZ
CSB-EP455711EYZ
CSB-BP455711EYZ
CSB-MP455711EYZ
CSB-EP455711EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis UPF0325 protein PMI2289 (PMI2289) CSB-YP455712EYZ
CSB-EP455712EYZ
CSB-BP455712EYZ
CSB-MP455712EYZ
CSB-EP455712EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Cell division protein FtsP (ftsP) CSB-YP455713EYZ
CSB-EP455713EYZ
CSB-BP455713EYZ
CSB-MP455713EYZ
CSB-EP455713EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 30S ribosomal protein S20 (rpsT) CSB-YP455714EYZ
CSB-EP455714EYZ
CSB-BP455714EYZ
CSB-MP455714EYZ
CSB-EP455714EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis UPF0178 protein PMI1258 (PMI1258) CSB-YP455715EYZ
CSB-EP455715EYZ
CSB-BP455715EYZ
CSB-MP455715EYZ
CSB-EP455715EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis UPF0631 protein C17orf108 homolog CSB-YP455716XBF
CSB-EP455716XBF
CSB-BP455716XBF
CSB-MP455716XBF
CSB-EP455716XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Ninja-family protein 1 CSB-YP455717ZAX
CSB-EP455717ZAX
CSB-BP455717ZAX
CSB-MP455717ZAX
CSB-EP455717ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 15-cis-zeta-carotene isomerase, chloroplastic, partial CSB-YP455718ZAX1
CSB-EP455718ZAX1
CSB-BP455718ZAX1
CSB-MP455718ZAX1
CSB-EP455718ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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