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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia cepacia UPF0317 protein BceJ2315_40370 (BceJ2315_40370) CSB-YP455659BXS
CSB-EP455659BXS
CSB-BP455659BXS
CSB-MP455659BXS
CSB-EP455659BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Deoxyribose-phosphate aldolase (deoC) CSB-YP455660BXS
CSB-EP455660BXS
CSB-BP455660BXS
CSB-MP455660BXS
CSB-EP455660BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis UvrABC system protein B (uvrB), partial CSB-YP455661EYZ
CSB-EP455661EYZ
CSB-BP455661EYZ
CSB-MP455661EYZ
CSB-EP455661EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP455662EYZ
CSB-EP455662EYZ
CSB-BP455662EYZ
CSB-MP455662EYZ
CSB-EP455662EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Cytidylate kinase (cmk) CSB-YP455663EYZ
CSB-EP455663EYZ
CSB-BP455663EYZ
CSB-MP455663EYZ
CSB-EP455663EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L25 (rplY) CSB-YP455664EYZ
CSB-EP455664EYZ
CSB-BP455664EYZ
CSB-MP455664EYZ
CSB-EP455664EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Integration host factor subunit alpha (ihfA) CSB-YP455665EYZ
CSB-EP455665EYZ
CSB-BP455665EYZ
CSB-MP455665EYZ
CSB-EP455665EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE), partial CSB-YP455666EYZ1
CSB-EP455666EYZ1
CSB-BP455666EYZ1
CSB-MP455666EYZ1
CSB-EP455666EYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Arginine--tRNA ligase (argS), partial CSB-YP455667EYZ
CSB-EP455667EYZ
CSB-BP455667EYZ
CSB-MP455667EYZ
CSB-EP455667EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP455668EYZ
CSB-EP455668EYZ
CSB-BP455668EYZ
CSB-MP455668EYZ
CSB-EP455668EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Transcriptional repressor NrdR (nrdR) CSB-YP455669EYZ
CSB-EP455669EYZ
CSB-BP455669EYZ
CSB-MP455669EYZ
CSB-EP455669EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Trigger factor (tig) CSB-YP455670EYZ
CSB-EP455670EYZ
CSB-BP455670EYZ
CSB-MP455670EYZ
CSB-EP455670EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Aspartate 1-decarboxylase (panD) CSB-YP455671EYZ
CSB-EP455671EYZ
CSB-BP455671EYZ
CSB-MP455671EYZ
CSB-EP455671EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP455672EYZ
CSB-EP455672EYZ
CSB-BP455672EYZ
CSB-MP455672EYZ
CSB-EP455672EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP455673EYZ
CSB-EP455673EYZ
CSB-BP455673EYZ
CSB-MP455673EYZ
CSB-EP455673EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis UPF0301 protein PMI0339 (PMI0339) CSB-YP455674EYZ
CSB-EP455674EYZ
CSB-BP455674EYZ
CSB-MP455674EYZ
CSB-EP455674EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Glutamate--cysteine ligase (gshA), partial CSB-YP455675EYZ
CSB-EP455675EYZ
CSB-BP455675EYZ
CSB-MP455675EYZ
CSB-EP455675EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Probable rRNA maturation factor (PMI0445) CSB-YP455676EYZ
CSB-EP455676EYZ
CSB-BP455676EYZ
CSB-MP455676EYZ
CSB-EP455676EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP455677EYZ
CSB-EP455677EYZ
CSB-BP455677EYZ
CSB-MP455677EYZ
CSB-EP455677EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP455678EYZ
CSB-EP455678EYZ
CSB-BP455678EYZ
CSB-MP455678EYZ
CSB-EP455678EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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