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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia cepacia Probable cytosol aminopeptidase (pepA), partial CSB-YP455639BXS
CSB-EP455639BXS
CSB-BP455639BXS
CSB-MP455639BXS
CSB-EP455639BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia 50S ribosomal protein L28 (rpmB) CSB-YP455640BXS
CSB-EP455640BXS
CSB-BP455640BXS
CSB-MP455640BXS
CSB-EP455640BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Adenylate kinase (adk) CSB-YP455641BXS
CSB-EP455641BXS
CSB-BP455641BXS
CSB-MP455641BXS
CSB-EP455641BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia tRNA (guanine-N (1)-)-methyltransferase CSB-YP455642BXS
CSB-EP455642BXS
CSB-BP455642BXS
CSB-MP455642BXS
CSB-EP455642BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia GTPase Der (der) CSB-YP455643BXS
CSB-EP455643BXS
CSB-BP455643BXS
CSB-MP455643BXS
CSB-EP455643BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Aspartate--tRNA ligase (aspS), partial CSB-YP455644BXS
CSB-EP455644BXS
CSB-BP455644BXS
CSB-MP455644BXS
CSB-EP455644BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Probable intracellular septation protein A (BceJ2315_19460), partial CSB-YP455645BXS1
CSB-EP455645BXS1
CSB-BP455645BXS1
CSB-MP455645BXS1
CSB-EP455645BXS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Serine--tRNA ligase (serS) CSB-YP455646BXS
CSB-EP455646BXS
CSB-BP455646BXS
CSB-MP455646BXS
CSB-EP455646BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP455647BXS
CSB-EP455647BXS
CSB-BP455647BXS
CSB-MP455647BXS
CSB-EP455647BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Lipid-A-disaccharide synthase (lpxB) CSB-YP455648BXS
CSB-EP455648BXS
CSB-BP455648BXS
CSB-MP455648BXS
CSB-EP455648BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP455649BXS
CSB-EP455649BXS
CSB-BP455649BXS
CSB-MP455649BXS
CSB-EP455649BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP455650BXS
CSB-EP455650BXS
CSB-BP455650BXS
CSB-MP455650BXS
CSB-EP455650BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Peptide chain release factor 2 (prfB) CSB-YP455651BXS
CSB-EP455651BXS
CSB-BP455651BXS
CSB-MP455651BXS
CSB-EP455651BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Bifunctional protein FolD (folD) CSB-YP455652BXS
CSB-EP455652BXS
CSB-BP455652BXS
CSB-MP455652BXS
CSB-EP455652BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP455653BXS
CSB-EP455653BXS
CSB-BP455653BXS
CSB-MP455653BXS
CSB-EP455653BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia 30S ribosomal protein S9 (rpsI) CSB-YP455654BXS
CSB-EP455654BXS
CSB-BP455654BXS
CSB-MP455654BXS
CSB-EP455654BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia ATP synthase epsilon chain (atpC) CSB-YP455655BXS
CSB-EP455655BXS
CSB-BP455655BXS
CSB-MP455655BXS
CSB-EP455655BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Lipase chaperone (lifO), partial CSB-YP455656BXS1
CSB-EP455656BXS1
CSB-BP455656BXS1
CSB-MP455656BXS1
CSB-EP455656BXS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP455657BXS
CSB-EP455657BXS
CSB-BP455657BXS
CSB-MP455657BXS
CSB-EP455657BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Coenzyme PQQ synthesis protein A (pqqA) CSB-YP455658BXS
CSB-EP455658BXS
CSB-BP455658BXS
CSB-MP455658BXS
CSB-EP455658BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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