Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. Ribosome maturation factor RimM (rimM) CSB-YP455899FQA
CSB-EP455899FQA
CSB-BP455899FQA
CSB-MP455899FQA
CSB-EP455899FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP455900FQA
CSB-EP455900FQA
CSB-BP455900FQA
CSB-MP455900FQA
CSB-EP455900FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP455901FQA
CSB-EP455901FQA
CSB-BP455901FQA
CSB-MP455901FQA
CSB-EP455901FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Sulfate adenylyltransferase (sat) CSB-YP455902FQA
CSB-EP455902FQA
CSB-BP455902FQA
CSB-MP455902FQA
CSB-EP455902FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. PKHD-type hydroxylase SYNPCC7002_A2658 (SYNPCC7002_A2658) CSB-YP455903FQA
CSB-EP455903FQA
CSB-BP455903FQA
CSB-MP455903FQA
CSB-EP455903FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP455904FQA
CSB-EP455904FQA
CSB-BP455904FQA
CSB-MP455904FQA
CSB-EP455904FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Adenylyl-sulfate kinase (cysC) CSB-YP455905FQA
CSB-EP455905FQA
CSB-BP455905FQA
CSB-MP455905FQA
CSB-EP455905FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP455906FQA
CSB-EP455906FQA
CSB-BP455906FQA
CSB-MP455906FQA
CSB-EP455906FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP455907FQA
CSB-EP455907FQA
CSB-BP455907FQA
CSB-MP455907FQA
CSB-EP455907FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. UPF0272 protein SYNPCC7002_A1710 (SYNPCC7002_A1710) CSB-YP455908FQA
CSB-EP455908FQA
CSB-BP455908FQA
CSB-MP455908FQA
CSB-EP455908FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S21 (rpsU) CSB-YP455909FQA
CSB-EP455909FQA
CSB-BP455909FQA
CSB-MP455909FQA
CSB-EP455909FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. NAD (P)H-quinone oxidoreductase subunit M CSB-YP455910FQA
CSB-EP455910FQA
CSB-BP455910FQA
CSB-MP455910FQA
CSB-EP455910FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP455911FQA
CSB-EP455911FQA
CSB-BP455911FQA
CSB-MP455911FQA
CSB-EP455911FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP455912FQA
CSB-EP455912FQA
CSB-BP455912FQA
CSB-MP455912FQA
CSB-EP455912FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S2 (rpsB) CSB-YP455913FQA
CSB-EP455913FQA
CSB-BP455913FQA
CSB-MP455913FQA
CSB-EP455913FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP455914EXM
CSB-EP455914EXM
CSB-BP455914EXM
CSB-MP455914EXM
CSB-EP455914EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP455915EXM
CSB-EP455915EXM
CSB-BP455915EXM
CSB-MP455915EXM
CSB-EP455915EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP455916EXM
CSB-EP455916EXM
CSB-BP455916EXM
CSB-MP455916EXM
CSB-EP455916EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius S-adenosylmethionine synthase (metK) CSB-YP455917EXM
CSB-EP455917EXM
CSB-BP455917EXM
CSB-MP455917EXM
CSB-EP455917EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L10 (rplJ) CSB-YP455918EXM
CSB-EP455918EXM
CSB-BP455918EXM
CSB-MP455918EXM
CSB-EP455918EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>