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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Stenotrophomonas maltophilia Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP456139FLX
CSB-EP456139FLX
CSB-BP456139FLX
CSB-MP456139FLX
CSB-EP456139FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Arginine--tRNA ligase (argS), partial CSB-YP456140FLX
CSB-EP456140FLX
CSB-BP456140FLX
CSB-MP456140FLX
CSB-EP456140FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia UvrABC system protein C (uvrC), partial CSB-YP456141FLX
CSB-EP456141FLX
CSB-BP456141FLX
CSB-MP456141FLX
CSB-EP456141FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP456142FLX
CSB-EP456142FLX
CSB-BP456142FLX
CSB-MP456142FLX
CSB-EP456142FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 50S ribosomal protein L9 (rplI) CSB-YP456143FLX
CSB-EP456143FLX
CSB-BP456143FLX
CSB-MP456143FLX
CSB-EP456143FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Protein RecA (recA) CSB-YP456144FLX
CSB-EP456144FLX
CSB-BP456144FLX
CSB-MP456144FLX
CSB-EP456144FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Aspartate 1-decarboxylase (panD) CSB-YP456145FLX
CSB-EP456145FLX
CSB-BP456145FLX
CSB-MP456145FLX
CSB-EP456145FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Phosphopantetheine adenylyltransferase (coaD) CSB-YP456146FLX
CSB-EP456146FLX
CSB-BP456146FLX
CSB-MP456146FLX
CSB-EP456146FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Homoserine O-acetyltransferase (metX) CSB-YP456147FLX
CSB-EP456147FLX
CSB-BP456147FLX
CSB-MP456147FLX
CSB-EP456147FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP456148FLX
CSB-EP456148FLX
CSB-BP456148FLX
CSB-MP456148FLX
CSB-EP456148FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia UPF0745 protein Smlt4554 (Smlt4554) CSB-YP456149FLX
CSB-EP456149FLX
CSB-BP456149FLX
CSB-MP456149FLX
CSB-EP456149FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP456150FLX
CSB-EP456150FLX
CSB-BP456150FLX
CSB-MP456150FLX
CSB-EP456150FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP456151FLX
CSB-EP456151FLX
CSB-BP456151FLX
CSB-MP456151FLX
CSB-EP456151FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP456152FLX
CSB-EP456152FLX
CSB-BP456152FLX
CSB-MP456152FLX
CSB-EP456152FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Dihydroorotate dehydrogenase (quinone) CSB-YP456153FLX
CSB-EP456153FLX
CSB-BP456153FLX
CSB-MP456153FLX
CSB-EP456153FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Acireductone dioxygenase (mtnD) CSB-YP456154FLX
CSB-EP456154FLX
CSB-BP456154FLX
CSB-MP456154FLX
CSB-EP456154FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Leucine--tRNA ligase (leuS), partial CSB-YP456155FLX
CSB-EP456155FLX
CSB-BP456155FLX
CSB-MP456155FLX
CSB-EP456155FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Glutamyl-tRNA reductase (hemA) CSB-YP456156FLX
CSB-EP456156FLX
CSB-BP456156FLX
CSB-MP456156FLX
CSB-EP456156FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 30S ribosomal protein S7 (rpsG) CSB-YP456157FLX
CSB-EP456157FLX
CSB-BP456157FLX
CSB-MP456157FLX
CSB-EP456157FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 50S ribosomal protein L16 (rplP) CSB-YP456158FLX
CSB-EP456158FLX
CSB-BP456158FLX
CSB-MP456158FLX
CSB-EP456158FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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