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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Stenotrophomonas maltophilia 50S ribosomal protein L24 (rplX) CSB-YP456159FLX
CSB-EP456159FLX
CSB-BP456159FLX
CSB-MP456159FLX
CSB-EP456159FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 30S ribosomal protein S4 (rpsD) CSB-YP456160FLX
CSB-EP456160FLX
CSB-BP456160FLX
CSB-MP456160FLX
CSB-EP456160FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP456161FLX
CSB-EP456161FLX
CSB-BP456161FLX
CSB-MP456161FLX
CSB-EP456161FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Probable transcriptional regulatory protein Smlt3713 (Smlt3713) CSB-YP456162FLX
CSB-EP456162FLX
CSB-BP456162FLX
CSB-MP456162FLX
CSB-EP456162FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia HPr kinase/phosphorylase (hprK) CSB-YP456163FLX
CSB-EP456163FLX
CSB-BP456163FLX
CSB-MP456163FLX
CSB-EP456163FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Large-conductance mechanosensitive channel (mscL), partial CSB-YP456164FLX1
CSB-EP456164FLX1
CSB-BP456164FLX1
CSB-MP456164FLX1
CSB-EP456164FLX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Adenylate kinase (adk) CSB-YP456165FLX
CSB-EP456165FLX
CSB-BP456165FLX
CSB-MP456165FLX
CSB-EP456165FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP456166FLX
CSB-EP456166FLX
CSB-BP456166FLX
CSB-MP456166FLX
CSB-EP456166FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia tRNA (guanine-N (1)-)-methyltransferase CSB-YP456167FLX
CSB-EP456167FLX
CSB-BP456167FLX
CSB-MP456167FLX
CSB-EP456167FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloferax volcanii S-layer biosynthesis glycosyltransferase AglI (aglI) CSB-YP456168HTT
CSB-EP456168HTT
CSB-BP456168HTT
CSB-MP456168HTT
CSB-EP456168HTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri DNA replication and repair protein recF (recF) CSB-YP456169LMV
CSB-EP456169LMV
CSB-BP456169LMV
CSB-MP456169LMV
CSB-EP456169LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Bifunctional protein pyrR (pyrR) CSB-YP456170LMV
CSB-EP456170LMV
CSB-BP456170LMV
CSB-MP456170LMV
CSB-EP456170LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP456171LMV
CSB-EP456171LMV
CSB-BP456171LMV
CSB-MP456171LMV
CSB-EP456171LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP456172LMV
CSB-EP456172LMV
CSB-BP456172LMV
CSB-MP456172LMV
CSB-EP456172LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Cysteine--tRNA ligase (cysS) CSB-YP456173LMV
CSB-EP456173LMV
CSB-BP456173LMV
CSB-MP456173LMV
CSB-EP456173LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP456174LMV
CSB-EP456174LMV
CSB-BP456174LMV
CSB-MP456174LMV
CSB-EP456174LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri HPr kinase/phosphorylase (hprK) CSB-YP456175LMV
CSB-EP456175LMV
CSB-BP456175LMV
CSB-MP456175LMV
CSB-EP456175LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP456176LMV
CSB-EP456176LMV
CSB-BP456176LMV
CSB-MP456176LMV
CSB-EP456176LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri 30S ribosomal protein S4 (rpsD) CSB-YP456177LMV
CSB-EP456177LMV
CSB-BP456177LMV
CSB-MP456177LMV
CSB-EP456177LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP456178LMV
CSB-EP456178LMV
CSB-BP456178LMV
CSB-MP456178LMV
CSB-EP456178LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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