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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Sulfurihydrogenibium sp. MutS2 protein (mutS2), partial CSB-YP456940FPP
CSB-EP456940FPP
CSB-BP456940FPP
CSB-MP456940FPP
CSB-EP456940FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. ATP synthase gamma chain (atpG) CSB-YP456941FPP
CSB-EP456941FPP
CSB-BP456941FPP
CSB-MP456941FPP
CSB-EP456941FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP456942FPP
CSB-EP456942FPP
CSB-BP456942FPP
CSB-MP456942FPP
CSB-EP456942FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 50S ribosomal protein L9 (rplI) CSB-YP456943FPP
CSB-EP456943FPP
CSB-BP456943FPP
CSB-MP456943FPP
CSB-EP456943FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Lipid-A-disaccharide synthase (lpxB) CSB-YP456944FPP
CSB-EP456944FPP
CSB-BP456944FPP
CSB-MP456944FPP
CSB-EP456944FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 50S ribosomal protein L19 (rplS) CSB-YP456945FPP
CSB-EP456945FPP
CSB-BP456945FPP
CSB-MP456945FPP
CSB-EP456945FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Probable transaldolase (tal) CSB-YP456946FPP
CSB-EP456946FPP
CSB-BP456946FPP
CSB-MP456946FPP
CSB-EP456946FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 50S ribosomal protein L17 (rplQ) CSB-YP456947FPP
CSB-EP456947FPP
CSB-BP456947FPP
CSB-MP456947FPP
CSB-EP456947FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 50S ribosomal protein L22 (rplV) CSB-YP456948FPP
CSB-EP456948FPP
CSB-BP456948FPP
CSB-MP456948FPP
CSB-EP456948FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 30S ribosomal protein S10 (rpsJ) CSB-YP456949FPP
CSB-EP456949FPP
CSB-BP456949FPP
CSB-MP456949FPP
CSB-EP456949FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 50S ribosomal protein L11 (rplK) CSB-YP456950FPP
CSB-EP456950FPP
CSB-BP456950FPP
CSB-MP456950FPP
CSB-EP456950FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Aspartate 1-decarboxylase (panD) CSB-YP456951FPP
CSB-EP456951FPP
CSB-BP456951FPP
CSB-MP456951FPP
CSB-EP456951FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Tryptophan synthase beta chain (trpB) CSB-YP456952FPP
CSB-EP456952FPP
CSB-BP456952FPP
CSB-MP456952FPP
CSB-EP456952FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP456953FPP
CSB-EP456953FPP
CSB-BP456953FPP
CSB-MP456953FPP
CSB-EP456953FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 50S ribosomal protein L28 (rpmB) CSB-YP456954FPP
CSB-EP456954FPP
CSB-BP456954FPP
CSB-MP456954FPP
CSB-EP456954FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Protein CrcB homolog (crcB), partial CSB-YP456955FPP1
CSB-EP456955FPP1
CSB-BP456955FPP1
CSB-MP456955FPP1
CSB-EP456955FPP1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Elongation factor P (efp) CSB-YP456956FPP
CSB-EP456956FPP
CSB-BP456956FPP
CSB-MP456956FPP
CSB-EP456956FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. 30S ribosomal protein S21 (rpsU) CSB-YP456957FPP
CSB-EP456957FPP
CSB-BP456957FPP
CSB-MP456957FPP
CSB-EP456957FPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Bifunctional protein FolD (folD) CSB-YP456958ENB
CSB-EP456958ENB
CSB-BP456958ENB
CSB-MP456958ENB
CSB-EP456958ENB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), partial CSB-YP456959ENB1
CSB-EP456959ENB1
CSB-BP456959ENB1
CSB-MP456959ENB1
CSB-EP456959ENB1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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