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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Erwinia tasmaniensis Lipid A palmitoyltransferase PagP (pagP) CSB-YP456960ENB
CSB-EP456960ENB
CSB-BP456960ENB
CSB-MP456960ENB
CSB-EP456960ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0271 protein ETA_23140 (ETA_23140) CSB-YP456961ENB
CSB-EP456961ENB
CSB-BP456961ENB
CSB-MP456961ENB
CSB-EP456961ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP456962ENB
CSB-EP456962ENB
CSB-BP456962ENB
CSB-MP456962ENB
CSB-EP456962ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP456963ENB
CSB-EP456963ENB
CSB-BP456963ENB
CSB-MP456963ENB
CSB-EP456963ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Alkanesulfonate monooxygenase (ssuD) CSB-YP456964ENB
CSB-EP456964ENB
CSB-BP456964ENB
CSB-MP456964ENB
CSB-EP456964ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis ATP synthase subunit c (atpE), partial CSB-YP456965ENB1
CSB-EP456965ENB1
CSB-BP456965ENB1
CSB-MP456965ENB1
CSB-EP456965ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Ribonuclease P protein component (rnpA) CSB-YP456966ENB
CSB-EP456966ENB
CSB-BP456966ENB
CSB-MP456966ENB
CSB-EP456966ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0059 membrane protein ETA_33770 (ETA_33770), partial CSB-YP456967ENB1
CSB-EP456967ENB1
CSB-BP456967ENB1
CSB-MP456967ENB1
CSB-EP456967ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Uncharacterized protein YjeA (yjeA) CSB-YP456968ENB
CSB-EP456968ENB
CSB-BP456968ENB
CSB-MP456968ENB
CSB-EP456968ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Adenylosuccinate synthetase (purA) CSB-YP456969ENB
CSB-EP456969ENB
CSB-BP456969ENB
CSB-MP456969ENB
CSB-EP456969ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0289 protein ETA_07970 (ETA_07970) CSB-YP456970ENB
CSB-EP456970ENB
CSB-BP456970ENB
CSB-MP456970ENB
CSB-EP456970ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Protein MraZ (mraZ) CSB-YP456971ENB
CSB-EP456971ENB
CSB-BP456971ENB
CSB-MP456971ENB
CSB-EP456971ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP456972ENB
CSB-EP456972ENB
CSB-BP456972ENB
CSB-MP456972ENB
CSB-EP456972ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0502 protein ETA_20480 (ETA_20480) CSB-YP456973ENB
CSB-EP456973ENB
CSB-BP456973ENB
CSB-MP456973ENB
CSB-EP456973ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Beta-hexosaminidase (nagZ) CSB-YP456974ENB
CSB-EP456974ENB
CSB-BP456974ENB
CSB-MP456974ENB
CSB-EP456974ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Protein nrdI (nrdI) CSB-YP456975ENB
CSB-EP456975ENB
CSB-BP456975ENB
CSB-MP456975ENB
CSB-EP456975ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP456976ENB
CSB-EP456976ENB
CSB-BP456976ENB
CSB-MP456976ENB
CSB-EP456976ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Dihydrodipicolinate synthase (dapA) CSB-YP456977ENB
CSB-EP456977ENB
CSB-BP456977ENB
CSB-MP456977ENB
CSB-EP456977ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP456978ENB
CSB-EP456978ENB
CSB-BP456978ENB
CSB-MP456978ENB
CSB-EP456978ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP456979ENB
CSB-EP456979ENB
CSB-BP456979ENB
CSB-MP456979ENB
CSB-EP456979ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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