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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium botulinum 3-dehydroquinate synthase (aroB) CSB-YP456920DUF
CSB-EP456920DUF
CSB-BP456920DUF
CSB-MP456920DUF
CSB-EP456920DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP456921DUF
CSB-EP456921DUF
CSB-BP456921DUF
CSB-MP456921DUF
CSB-EP456921DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP synthase epsilon chain (atpC) CSB-YP456922DUF
CSB-EP456922DUF
CSB-BP456922DUF
CSB-MP456922DUF
CSB-EP456922DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP456923DUF
CSB-EP456923DUF
CSB-BP456923DUF
CSB-MP456923DUF
CSB-EP456923DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glycogen synthase (glgA) CSB-YP456924DUF
CSB-EP456924DUF
CSB-BP456924DUF
CSB-MP456924DUF
CSB-EP456924DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum GTPase obg (obg) CSB-YP456925DUF
CSB-EP456925DUF
CSB-BP456925DUF
CSB-MP456925DUF
CSB-EP456925DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glucosamine-6-phosphate deaminase (nagB) CSB-YP456926DUF
CSB-EP456926DUF
CSB-BP456926DUF
CSB-MP456926DUF
CSB-EP456926DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Adenylosuccinate synthetase (purA) CSB-YP456927DUF
CSB-EP456927DUF
CSB-BP456927DUF
CSB-MP456927DUF
CSB-EP456927DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP456928DUF
CSB-EP456928DUF
CSB-BP456928DUF
CSB-MP456928DUF
CSB-EP456928DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable rRNA maturation factor (CLH_0871) CSB-YP456929DUF
CSB-EP456929DUF
CSB-BP456929DUF
CSB-MP456929DUF
CSB-EP456929DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP456930DUF
CSB-EP456930DUF
CSB-BP456930DUF
CSB-MP456930DUF
CSB-EP456930DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum FMN-dependent NADH-azoreductase (azoR) CSB-YP456931DUF
CSB-EP456931DUF
CSB-BP456931DUF
CSB-MP456931DUF
CSB-EP456931DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Alanine--tRNA ligase (alaS), partial CSB-YP456932DUF
CSB-EP456932DUF
CSB-BP456932DUF
CSB-MP456932DUF
CSB-EP456932DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Sugar fermentation stimulation protein homolog (sfsA) CSB-YP456933DUF
CSB-EP456933DUF
CSB-BP456933DUF
CSB-MP456933DUF
CSB-EP456933DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L28 (rpmB) CSB-YP456934DUF
CSB-EP456934DUF
CSB-BP456934DUF
CSB-MP456934DUF
CSB-EP456934DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP456935DUF
CSB-EP456935DUF
CSB-BP456935DUF
CSB-MP456935DUF
CSB-EP456935DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP456936DUF
CSB-EP456936DUF
CSB-BP456936DUF
CSB-MP456936DUF
CSB-EP456936DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Catalase-peroxidase (katG), partial CSB-YP456937DUF
CSB-EP456937DUF
CSB-BP456937DUF
CSB-MP456937DUF
CSB-EP456937DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L20 (rplT) CSB-YP456938DUF
CSB-EP456938DUF
CSB-BP456938DUF
CSB-MP456938DUF
CSB-EP456938DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. Glutamate--tRNA ligase (gltX) CSB-YP456939FPP
CSB-EP456939FPP
CSB-BP456939FPP
CSB-MP456939FPP
CSB-EP456939FPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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