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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype IB DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP460361YAW
CSB-EP460361YAW
CSB-BP460361YAW
CSB-MP460361YAW
CSB-EP460361YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Flavoprotein wrbA (wrbA) CSB-YP460362YAW
CSB-EP460362YAW
CSB-BP460362YAW
CSB-MP460362YAW
CSB-EP460362YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB HTH-type transcriptional activator rhaS (rhaS) CSB-YP460363YAW
CSB-EP460363YAW
CSB-BP460363YAW
CSB-MP460363YAW
CSB-EP460363YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Fumarate reductase subunit C (frdC), partial CSB-YP460364YAW1
CSB-EP460364YAW1
CSB-BP460364YAW1
CSB-MP460364YAW1
CSB-EP460364YAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB N-succinylglutamate 5-semialdehyde dehydrogenase (astD), partial CSB-YP460365YAW
CSB-EP460365YAW
CSB-BP460365YAW
CSB-MP460365YAW
CSB-EP460365YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP460366YAW1
CSB-EP460366YAW1
CSB-BP460366YAW1
CSB-MP460366YAW1
CSB-EP460366YAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L9 (rplI) CSB-YP460367YAW
CSB-EP460367YAW
CSB-BP460367YAW
CSB-MP460367YAW
CSB-EP460367YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB GTPase obg (obg) CSB-YP460368YAW
CSB-EP460368YAW
CSB-BP460368YAW
CSB-MP460368YAW
CSB-EP460368YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Outer-membrane lipoprotein lolB (lolB) CSB-YP460369YAW
CSB-EP460369YAW
CSB-BP460369YAW
CSB-MP460369YAW
CSB-EP460369YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB tRNA (mo5U34)-methyltransferase CSB-YP460370YAW
CSB-EP460370YAW
CSB-BP460370YAW
CSB-MP460370YAW
CSB-EP460370YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Spermidine export protein MdtI (mdtI), partial CSB-YP460371YAW1
CSB-EP460371YAW1
CSB-BP460371YAW1
CSB-MP460371YAW1
CSB-EP460371YAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Uncharacterized aldolase lsrF (lsrF) CSB-YP460372YAW
CSB-EP460372YAW
CSB-BP460372YAW
CSB-MP460372YAW
CSB-EP460372YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Peptide chain release factor 3 (prfC), partial CSB-YP460373YAW
CSB-EP460373YAW
CSB-BP460373YAW
CSB-MP460373YAW
CSB-EP460373YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Probable phosphoglycerate mutase GpmB (gpmB) CSB-YP460374YAW
CSB-EP460374YAW
CSB-BP460374YAW
CSB-MP460374YAW
CSB-EP460374YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Na (+)/H (+) antiporter nhaA, partial CSB-YP460375YAW
CSB-EP460375YAW
CSB-BP460375YAW
CSB-MP460375YAW
CSB-EP460375YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0745 protein YPTS_2123 (YPTS_2123) CSB-YP460376YAW
CSB-EP460376YAW
CSB-BP460376YAW
CSB-MP460376YAW
CSB-EP460376YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB D-amino acid dehydrogenase small subunit (dadA) CSB-YP460377YAW
CSB-EP460377YAW
CSB-BP460377YAW
CSB-MP460377YAW
CSB-EP460377YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0102 protein YPTS_3679 (YPTS_3679) CSB-YP460378YAW
CSB-EP460378YAW
CSB-BP460378YAW
CSB-MP460378YAW
CSB-EP460378YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP460379YAW
CSB-EP460379YAW
CSB-BP460379YAW
CSB-MP460379YAW
CSB-EP460379YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB p-hydroxybenzoic acid efflux pump subunit AaeB (aaeB), partial CSB-YP460380YAW
CSB-EP460380YAW
CSB-BP460380YAW
CSB-MP460380YAW
CSB-EP460380YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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