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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Mycobacterium marinum Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP462062MVS
CSB-EP462062MVS
CSB-BP462062MVS
CSB-MP462062MVS
CSB-EP462062MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP462063MVS
CSB-EP462063MVS
CSB-BP462063MVS
CSB-MP462063MVS
CSB-EP462063MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP462064MVS
CSB-EP462064MVS
CSB-BP462064MVS
CSB-MP462064MVS
CSB-EP462064MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Anthranilate phosphoribosyltransferase (trpD) CSB-YP462065MVS
CSB-EP462065MVS
CSB-BP462065MVS
CSB-MP462065MVS
CSB-EP462065MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_4850 (MMAR_4850) CSB-YP462066MVS
CSB-EP462066MVS
CSB-BP462066MVS
CSB-MP462066MVS
CSB-EP462066MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP462067MVS
CSB-EP462067MVS
CSB-BP462067MVS
CSB-MP462067MVS
CSB-EP462067MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP462068MVS
CSB-EP462068MVS
CSB-BP462068MVS
CSB-MP462068MVS
CSB-EP462068MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 30S ribosomal protein S18 1 (rpsR1) CSB-YP462069MVS
CSB-EP462069MVS
CSB-BP462069MVS
CSB-MP462069MVS
CSB-EP462069MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Glutamate--tRNA ligase (gltX) CSB-YP462070MVS
CSB-EP462070MVS
CSB-BP462070MVS
CSB-MP462070MVS
CSB-EP462070MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Uncharacterized oxidoreductase MMAR_1744 (MMAR_1744) CSB-YP462071MVS
CSB-EP462071MVS
CSB-BP462071MVS
CSB-MP462071MVS
CSB-EP462071MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Cysteine--tRNA ligase (cysS) CSB-YP462072MVS
CSB-EP462072MVS
CSB-BP462072MVS
CSB-MP462072MVS
CSB-EP462072MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L19 (rplS) CSB-YP462073MVS
CSB-EP462073MVS
CSB-BP462073MVS
CSB-MP462073MVS
CSB-EP462073MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Haloalkane dehalogenase (dhmA) CSB-YP462074MVS
CSB-EP462074MVS
CSB-BP462074MVS
CSB-MP462074MVS
CSB-EP462074MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Diaminopimelate epimerase (dapF) CSB-YP462075MVS
CSB-EP462075MVS
CSB-BP462075MVS
CSB-MP462075MVS
CSB-EP462075MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_0356 (MMAR_0356) CSB-YP462076MVS
CSB-EP462076MVS
CSB-BP462076MVS
CSB-MP462076MVS
CSB-EP462076MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum GTPase obg (obg) CSB-YP462077MVS
CSB-EP462077MVS
CSB-BP462077MVS
CSB-MP462077MVS
CSB-EP462077MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP462078MVS
CSB-EP462078MVS
CSB-BP462078MVS
CSB-MP462078MVS
CSB-EP462078MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Elongation factor P (efp) CSB-YP462079MVS
CSB-EP462079MVS
CSB-BP462079MVS
CSB-MP462079MVS
CSB-EP462079MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum ESX-1 secretion-associated protein EspB (espB) CSB-YP462080MVS
CSB-EP462080MVS
CSB-BP462080MVS
CSB-MP462080MVS
CSB-EP462080MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP462081MVS
CSB-EP462081MVS
CSB-BP462081MVS
CSB-MP462081MVS
CSB-EP462081MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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