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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia ambifaria Urease accessory protein UreF (ureF) CSB-YP461861BXP
CSB-EP461861BXP
CSB-BP461861BXP
CSB-MP461861BXP
CSB-EP461861BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Serine--tRNA ligase (serS) CSB-YP461862BXP
CSB-EP461862BXP
CSB-BP461862BXP
CSB-MP461862BXP
CSB-EP461862BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Aspartate 1-decarboxylase (panD) CSB-YP461863BXP
CSB-EP461863BXP
CSB-BP461863BXP
CSB-MP461863BXP
CSB-EP461863BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria UPF0758 protein BamMC406_2419 (BamMC406_2419) CSB-YP461864BXP
CSB-EP461864BXP
CSB-BP461864BXP
CSB-MP461864BXP
CSB-EP461864BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 3-deoxy-manno-octulosonate cytidylyltransferase 1 (kdsB1) CSB-YP461865BXP
CSB-EP461865BXP
CSB-BP461865BXP
CSB-MP461865BXP
CSB-EP461865BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Elongation factor P (efp) CSB-YP461866BXP
CSB-EP461866BXP
CSB-BP461866BXP
CSB-MP461866BXP
CSB-EP461866BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP461867BXP
CSB-EP461867BXP
CSB-BP461867BXP
CSB-MP461867BXP
CSB-EP461867BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP461868BXP
CSB-EP461868BXP
CSB-BP461868BXP
CSB-MP461868BXP
CSB-EP461868BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi 30S ribosomal protein S17 (rpsQ) CSB-YP461869MTC
CSB-EP461869MTC
CSB-BP461869MTC
CSB-MP461869MTC
CSB-EP461869MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi 50S ribosomal protein L30 (rpmD) CSB-YP461870MTC
CSB-EP461870MTC
CSB-BP461870MTC
CSB-MP461870MTC
CSB-EP461870MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi Sulfate adenylyltransferase subunit 1 (cysN) CSB-YP461871MTC
CSB-EP461871MTC
CSB-BP461871MTC
CSB-MP461871MTC
CSB-EP461871MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi Membrane protein insertase YidC (yidC), partial CSB-YP461872MTC1
CSB-EP461872MTC1
CSB-BP461872MTC1
CSB-MP461872MTC1
CSB-EP461872MTC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP461873MTC
CSB-EP461873MTC
CSB-BP461873MTC
CSB-MP461873MTC
CSB-EP461873MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi Trigger factor (tig) CSB-YP461874MTC
CSB-EP461874MTC
CSB-BP461874MTC
CSB-MP461874MTC
CSB-EP461874MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi Cobyric acid synthase (cobQ) CSB-YP461875MTC
CSB-EP461875MTC
CSB-BP461875MTC
CSB-MP461875MTC
CSB-EP461875MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP461876MTC
CSB-EP461876MTC
CSB-BP461876MTC
CSB-MP461876MTC
CSB-EP461876MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB (rutB) CSB-YP461877MTC
CSB-EP461877MTC
CSB-BP461877MTC
CSB-MP461877MTC
CSB-EP461877MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi Urease accessory protein UreD 1 (ureD1) CSB-YP461878MTC
CSB-EP461878MTC
CSB-BP461878MTC
CSB-MP461878MTC
CSB-EP461878MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP461879MTC
CSB-EP461879MTC
CSB-BP461879MTC
CSB-MP461879MTC
CSB-EP461879MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi 50S ribosomal protein L1 (rplA) CSB-YP461880MTC
CSB-EP461880MTC
CSB-BP461880MTC
CSB-MP461880MTC
CSB-EP461880MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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