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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella boydii serotype 18 UPF0289 protein yacF (yacF) CSB-YP462662SYZ
CSB-EP462662SYZ
CSB-BP462662SYZ
CSB-MP462662SYZ
CSB-EP462662SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Electron transport complex protein RnfC (rnfC), partial CSB-YP462663SYZ
CSB-EP462663SYZ
CSB-BP462663SYZ
CSB-MP462663SYZ
CSB-EP462663SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Cysteine desulfuration protein sufE (sufE) CSB-YP462664SYZ
CSB-EP462664SYZ
CSB-BP462664SYZ
CSB-MP462664SYZ
CSB-EP462664SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 DnaA initiator-associating protein diaA (diaA) CSB-YP462665SYZ
CSB-EP462665SYZ
CSB-BP462665SYZ
CSB-MP462665SYZ
CSB-EP462665SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L30 (rpmD) CSB-YP462666SYZ
CSB-EP462666SYZ
CSB-BP462666SYZ
CSB-MP462666SYZ
CSB-EP462666SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L14 (rplN) CSB-YP462667SYZ
CSB-EP462667SYZ
CSB-BP462667SYZ
CSB-MP462667SYZ
CSB-EP462667SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L3 (rplC) CSB-YP462668SYZ
CSB-EP462668SYZ
CSB-BP462668SYZ
CSB-MP462668SYZ
CSB-EP462668SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Sulfurtransferase TusD (tusD) CSB-YP462669SYZ
CSB-EP462669SYZ
CSB-BP462669SYZ
CSB-MP462669SYZ
CSB-EP462669SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Pantothenate synthetase (panC) CSB-YP462670SYZ
CSB-EP462670SYZ
CSB-BP462670SYZ
CSB-MP462670SYZ
CSB-EP462670SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP462671SYZ
CSB-EP462671SYZ
CSB-BP462671SYZ
CSB-MP462671SYZ
CSB-EP462671SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0061 protein ydiU (ydiU) CSB-YP462672SYZ
CSB-EP462672SYZ
CSB-BP462672SYZ
CSB-MP462672SYZ
CSB-EP462672SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 NH (3)-dependent NAD (+) synthetase CSB-YP462673SYZ
CSB-EP462673SYZ
CSB-BP462673SYZ
CSB-MP462673SYZ
CSB-EP462673SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP462674SYZ
CSB-EP462674SYZ
CSB-BP462674SYZ
CSB-MP462674SYZ
CSB-EP462674SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 3-dehydroquinate synthase (aroB) CSB-YP462675SYZ
CSB-EP462675SYZ
CSB-BP462675SYZ
CSB-MP462675SYZ
CSB-EP462675SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 RNA 3'-terminal phosphate cyclase (rtcA) CSB-YP462676SYZ
CSB-EP462676SYZ
CSB-BP462676SYZ
CSB-MP462676SYZ
CSB-EP462676SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Phosphoheptose isomerase (gmhA) CSB-YP462677SYZ
CSB-EP462677SYZ
CSB-BP462677SYZ
CSB-MP462677SYZ
CSB-EP462677SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0345 protein YaiE (yaiE) CSB-YP462678SYZ
CSB-EP462678SYZ
CSB-BP462678SYZ
CSB-MP462678SYZ
CSB-EP462678SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP462679SYZ
CSB-EP462679SYZ
CSB-BP462679SYZ
CSB-MP462679SYZ
CSB-EP462679SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Nickel-responsive regulator (nikR) CSB-YP462680SYZ
CSB-EP462680SYZ
CSB-BP462680SYZ
CSB-MP462680SYZ
CSB-EP462680SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Cytoplasmic trehalase (treF), partial CSB-YP462681SYZ
CSB-EP462681SYZ
CSB-BP462681SYZ
CSB-MP462681SYZ
CSB-EP462681SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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