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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycobacterium marinum Thiazole synthase (thiG) CSB-YP462082MVS
CSB-EP462082MVS
CSB-BP462082MVS
CSB-MP462082MVS
CSB-EP462082MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP462083MVS
CSB-EP462083MVS
CSB-BP462083MVS
CSB-MP462083MVS
CSB-EP462083MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum ATP synthase epsilon chain (atpC) CSB-YP462084MVS
CSB-EP462084MVS
CSB-BP462084MVS
CSB-MP462084MVS
CSB-EP462084MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum ATP synthase subunit c (atpE), partial CSB-YP462085MVS1
CSB-EP462085MVS1
CSB-BP462085MVS1
CSB-MP462085MVS1
CSB-EP462085MVS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Deoxyribose-phosphate aldolase (deoC) CSB-YP462086MVS
CSB-EP462086MVS
CSB-BP462086MVS
CSB-MP462086MVS
CSB-EP462086MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Indole-3-glycerol phosphate synthase (trpC) CSB-YP462087MVS
CSB-EP462087MVS
CSB-BP462087MVS
CSB-MP462087MVS
CSB-EP462087MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP462088MVS
CSB-EP462088MVS
CSB-BP462088MVS
CSB-MP462088MVS
CSB-EP462088MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Probable endonuclease 4 (nfo) CSB-YP462089MVS
CSB-EP462089MVS
CSB-BP462089MVS
CSB-MP462089MVS
CSB-EP462089MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum 50S ribosomal protein L23 (rplW) CSB-YP462090MVS
CSB-EP462090MVS
CSB-BP462090MVS
CSB-MP462090MVS
CSB-EP462090MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Urease accessory protein UreF (ureF) CSB-YP462091MVS
CSB-EP462091MVS
CSB-BP462091MVS
CSB-MP462091MVS
CSB-EP462091MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Threonine--tRNA ligase (thrS), partial CSB-YP462092AWO
CSB-EP462092AWO
CSB-BP462092AWO
CSB-MP462092AWO
CSB-EP462092AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Hydroxyethylthiazole kinase (thiM) CSB-YP462093AWO
CSB-EP462093AWO
CSB-BP462093AWO
CSB-MP462093AWO
CSB-EP462093AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP462094AWO1
CSB-EP462094AWO1
CSB-BP462094AWO1
CSB-MP462094AWO1
CSB-EP462094AWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Glutamyl-tRNA reductase (hemA) CSB-YP462095AWO
CSB-EP462095AWO
CSB-BP462095AWO
CSB-MP462095AWO
CSB-EP462095AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Acetylglutamate kinase (argB) CSB-YP462096AWO
CSB-EP462096AWO
CSB-BP462096AWO
CSB-MP462096AWO
CSB-EP462096AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Probable L-aspartate dehydrogenase (nadX) CSB-YP462097AWO
CSB-EP462097AWO
CSB-BP462097AWO
CSB-MP462097AWO
CSB-EP462097AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 5'-nucleotidase surE (surE) CSB-YP462098AWO
CSB-EP462098AWO
CSB-BP462098AWO
CSB-MP462098AWO
CSB-EP462098AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Urease accessory protein UreG (ureG) CSB-YP462099AWO
CSB-EP462099AWO
CSB-BP462099AWO
CSB-MP462099AWO
CSB-EP462099AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii GTPase obg (obg) CSB-YP462100AWO
CSB-EP462100AWO
CSB-BP462100AWO
CSB-MP462100AWO
CSB-EP462100AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Cysteine--tRNA ligase (cysS) CSB-YP462101AWO
CSB-EP462101AWO
CSB-BP462101AWO
CSB-MP462101AWO
CSB-EP462101AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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