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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xylella fastidiosa Lipase chaperone (lifO), partial CSB-YP462142XBN1
CSB-EP462142XBN1
CSB-BP462142XBN1
CSB-MP462142XBN1
CSB-EP462142XBN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP462143XBN
CSB-EP462143XBN
CSB-BP462143XBN
CSB-MP462143XBN
CSB-EP462143XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Cell division topological specificity factor (minE) CSB-YP462144XBN
CSB-EP462144XBN
CSB-BP462144XBN
CSB-MP462144XBN
CSB-EP462144XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP462145XBN
CSB-EP462145XBN
CSB-BP462145XBN
CSB-MP462145XBN
CSB-EP462145XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 8-amino-7-oxononanoate synthase (bioF) CSB-YP462146XBN
CSB-EP462146XBN
CSB-BP462146XBN
CSB-MP462146XBN
CSB-EP462146XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UPF0042 nucleotide-binding protein XfasM23_0667 (XfasM23_0667) CSB-YP462147XBN
CSB-EP462147XBN
CSB-BP462147XBN
CSB-MP462147XBN
CSB-EP462147XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Threonine--tRNA ligase (thrS), partial CSB-YP462148XBN
CSB-EP462148XBN
CSB-BP462148XBN
CSB-MP462148XBN
CSB-EP462148XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S6 (rpsF) CSB-YP462149XBN
CSB-EP462149XBN
CSB-BP462149XBN
CSB-MP462149XBN
CSB-EP462149XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UPF0176 protein XfasM23_2090 (XfasM23_2090) CSB-YP462150XBN
CSB-EP462150XBN
CSB-BP462150XBN
CSB-MP462150XBN
CSB-EP462150XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Protein CrcB homolog (crcB), partial CSB-YP462151XBN1
CSB-EP462151XBN1
CSB-BP462151XBN1
CSB-MP462151XBN1
CSB-EP462151XBN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP462152XBN
CSB-EP462152XBN
CSB-BP462152XBN
CSB-MP462152XBN
CSB-EP462152XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L7/L12 (rplL) CSB-YP462153XBN
CSB-EP462153XBN
CSB-BP462153XBN
CSB-MP462153XBN
CSB-EP462153XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L25 (rplY) CSB-YP462154XBN
CSB-EP462154XBN
CSB-BP462154XBN
CSB-MP462154XBN
CSB-EP462154XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L31 type B (rpmE2) CSB-YP462155XBN
CSB-EP462155XBN
CSB-BP462155XBN
CSB-MP462155XBN
CSB-EP462155XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa GTP cyclohydrolase folE2 (folE2) CSB-YP462156XBN
CSB-EP462156XBN
CSB-BP462156XBN
CSB-MP462156XBN
CSB-EP462156XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Tyrosine--tRNA ligase (tyrS) CSB-YP462157BSP
CSB-EP462157BSP
CSB-BP462157BSP
CSB-MP462157BSP
CSB-EP462157BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 50S ribosomal protein L33 (rpmG) CSB-YP462158BSP
CSB-EP462158BSP
CSB-BP462158BSP
CSB-MP462158BSP
CSB-EP462158BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Formate--tetrahydrofolate ligase (fhs), partial CSB-YP462159BSP
CSB-EP462159BSP
CSB-BP462159BSP
CSB-MP462159BSP
CSB-EP462159BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 50S ribosomal protein L31 (rpmE) CSB-YP462160BSP
CSB-EP462160BSP
CSB-BP462160BSP
CSB-MP462160BSP
CSB-EP462160BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 30S ribosomal protein S15 (rpsO) CSB-YP462161BSP
CSB-EP462161BSP
CSB-BP462161BSP
CSB-MP462161BSP
CSB-EP462161BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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