Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype IB Peptidase T (pepT) CSB-YP462342YAW
CSB-EP462342YAW
CSB-BP462342YAW
CSB-MP462342YAW
CSB-EP462342YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Xylose isomerase (xylA) CSB-YP462343YAW
CSB-EP462343YAW
CSB-BP462343YAW
CSB-MP462343YAW
CSB-EP462343YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP462344YAW
CSB-EP462344YAW
CSB-BP462344YAW
CSB-MP462344YAW
CSB-EP462344YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB L-seryl-tRNA (Sec) selenium transferase CSB-YP462345YAW
CSB-EP462345YAW
CSB-BP462345YAW
CSB-MP462345YAW
CSB-EP462345YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP462346YAW
CSB-EP462346YAW
CSB-BP462346YAW
CSB-MP462346YAW
CSB-EP462346YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP462347YAW1
CSB-EP462347YAW1
CSB-BP462347YAW1
CSB-MP462347YAW1
CSB-EP462347YAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Acetate kinase (ackA) CSB-YP462348YAW
CSB-EP462348YAW
CSB-BP462348YAW
CSB-MP462348YAW
CSB-EP462348YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L34 (rpmH) CSB-YP462349YAW
CSB-EP462349YAW
CSB-BP462349YAW
CSB-MP462349YAW
CSB-EP462349YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Glutamine--tRNA ligase (glnS), partial CSB-YP462350YAW
CSB-EP462350YAW
CSB-BP462350YAW
CSB-MP462350YAW
CSB-EP462350YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Quinolinate synthase A (nadA) CSB-YP462351YAW
CSB-EP462351YAW
CSB-BP462351YAW
CSB-MP462351YAW
CSB-EP462351YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP462352YAW
CSB-EP462352YAW
CSB-BP462352YAW
CSB-MP462352YAW
CSB-EP462352YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Glutamate--tRNA ligase (gltX) CSB-YP462353YAW
CSB-EP462353YAW
CSB-BP462353YAW
CSB-MP462353YAW
CSB-EP462353YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB N-acetylmannosamine kinase (nanK) CSB-YP462354YAW
CSB-EP462354YAW
CSB-BP462354YAW
CSB-MP462354YAW
CSB-EP462354YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP462355YAW
CSB-EP462355YAW
CSB-BP462355YAW
CSB-MP462355YAW
CSB-EP462355YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0352 protein YPTS_1389 (YPTS_1389) CSB-YP462356YAW
CSB-EP462356YAW
CSB-BP462356YAW
CSB-MP462356YAW
CSB-EP462356YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Uracil phosphoribosyltransferase (upp) CSB-YP462357YAW
CSB-EP462357YAW
CSB-BP462357YAW
CSB-MP462357YAW
CSB-EP462357YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP462358YAW
CSB-EP462358YAW
CSB-BP462358YAW
CSB-MP462358YAW
CSB-EP462358YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Putative transport protein YPTS_1454 (YPTS_1454), partial CSB-YP462359YAW
CSB-EP462359YAW
CSB-BP462359YAW
CSB-MP462359YAW
CSB-EP462359YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Serine--tRNA ligase (serS) CSB-YP462360YAW
CSB-EP462360YAW
CSB-BP462360YAW
CSB-MP462360YAW
CSB-EP462360YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Elongation factor 4 (lepA), partial CSB-YP462361YAW
CSB-EP462361YAW
CSB-BP462361YAW
CSB-MP462361YAW
CSB-EP462361YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>