Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Helicobacter pylori Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP462742HUZ
CSB-EP462742HUZ
CSB-BP462742HUZ
CSB-MP462742HUZ
CSB-EP462742HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Dihydroorotate dehydrogenase (quinone) CSB-YP462743HUZ
CSB-EP462743HUZ
CSB-BP462743HUZ
CSB-MP462743HUZ
CSB-EP462743HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori GTPase Der (der) CSB-YP462744HUZ
CSB-EP462744HUZ
CSB-BP462744HUZ
CSB-MP462744HUZ
CSB-EP462744HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP462745HUZ
CSB-EP462745HUZ
CSB-BP462745HUZ
CSB-MP462745HUZ
CSB-EP462745HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP462746HUZ
CSB-EP462746HUZ
CSB-BP462746HUZ
CSB-MP462746HUZ
CSB-EP462746HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L31 (rpmE) CSB-YP462747HUZ
CSB-EP462747HUZ
CSB-BP462747HUZ
CSB-MP462747HUZ
CSB-EP462747HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP462748HUZ
CSB-EP462748HUZ
CSB-BP462748HUZ
CSB-MP462748HUZ
CSB-EP462748HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP462749HUZ
CSB-EP462749HUZ
CSB-BP462749HUZ
CSB-MP462749HUZ
CSB-EP462749HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP462750HUZ
CSB-EP462750HUZ
CSB-BP462750HUZ
CSB-MP462750HUZ
CSB-EP462750HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Ribosome maturation factor RimM (rimM) CSB-YP462751HUZ
CSB-EP462751HUZ
CSB-BP462751HUZ
CSB-MP462751HUZ
CSB-EP462751HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Alanine--tRNA ligase (alaS), partial CSB-YP462752HUZ
CSB-EP462752HUZ
CSB-BP462752HUZ
CSB-MP462752HUZ
CSB-EP462752HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Tryptophan synthase alpha chain (trpA) CSB-YP462753HUZ
CSB-EP462753HUZ
CSB-BP462753HUZ
CSB-MP462753HUZ
CSB-EP462753HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 30S ribosomal protein S13 (rpsM) CSB-YP462754HUZ
CSB-EP462754HUZ
CSB-BP462754HUZ
CSB-MP462754HUZ
CSB-EP462754HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L2 (rplB) CSB-YP462755HUZ
CSB-EP462755HUZ
CSB-BP462755HUZ
CSB-MP462755HUZ
CSB-EP462755HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP462756HUZ
CSB-EP462756HUZ
CSB-BP462756HUZ
CSB-MP462756HUZ
CSB-EP462756HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Putative membrane protein insertion efficiency factor (HPSH_07415) CSB-YP462757HUZ
CSB-EP462757HUZ
CSB-BP462757HUZ
CSB-MP462757HUZ
CSB-EP462757HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP462758HUZ
CSB-EP462758HUZ
CSB-BP462758HUZ
CSB-MP462758HUZ
CSB-EP462758HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP462759HUZ
CSB-EP462759HUZ
CSB-BP462759HUZ
CSB-MP462759HUZ
CSB-EP462759HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP462760HUZ
CSB-EP462760HUZ
CSB-BP462760HUZ
CSB-MP462760HUZ
CSB-EP462760HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Urease subunit beta (ureB), partial CSB-YP462761HUZ
CSB-EP462761HUZ
CSB-BP462761HUZ
CSB-MP462761HUZ
CSB-EP462761HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>